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Pizarro MD, Mediavilla MG, Quintana AB, et al. Performance of cold-preserved rat liver Microorgans as the biological component of a simplified prototype model of bioartificial liver. World J Hepatol. 2016;8(33):1442-1451doi: 10.4254/wjh.v8.i33.1442.
Pizarro, M. D., Mediavilla, M. G., Quintana, A. B., Scandizzi, �. �. L., Rodriguez, J. V., & Mamprin, M. E. (2016). Performance of cold-preserved rat liver Microorgans as the biological component of a simplified prototype model of bioartificial liver. World journal of hepatology, 8(33), 1442-1451. https://doi.org/10.4254/wjh.v8.i33.1442
Pizarro, María Dolores, et al. "Performance of cold-preserved rat liver Microorgans as the biological component of a simplified prototype model of bioartificial liver." World journal of hepatology vol. 8,33 (2016): 1442-1451. doi: https://doi.org/10.4254/wjh.v8.i33.1442
Pizarro MD, Mediavilla MG, Quintana AB, Scandizzi ÁL, Rodriguez JV, Mamprin ME. Performance of cold-preserved rat liver Microorgans as the biological component of a simplified prototype model of bioartificial liver. World J Hepatol. 2016 Nov 28;8(33):1442-1451. doi: 10.4254/wjh.v8.i33.1442. PMID: 27957242; PMCID: PMC5124715.
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World J Hepatol. 2016 Nov 28;8(33):1442-1451. doi: 10.4254/wjh.v8.i33.1442.

Performance of cold-preserved rat liver Microorgans as the biological component of a simplified prototype model of bioartificial liver.

World journal of hepatology

María Dolores Pizarro, María Gabriela Mediavilla, Alejandra Beatriz Quintana, Ángel Luis Scandizzi, Joaquín Valentín Rodriguez, María Eugenia Mamprin

Affiliations

  1. María Dolores Pizarro, Joaquín Valentín Rodríguez, Centro Binacional de Criobiología Clínica y Aplicada, Rosario 2000, Argentina.

PMID: 27957242 PMCID: PMC5124715 DOI: 10.4254/wjh.v8.i33.1442

Abstract

AIM: To develop a simplified bioartificial liver (BAL) device prototype, suitable to use freshly and preserved liver Microorgans (LMOs) as biological component.

METHODS: The system consists of 140 capillary fibers through which goat blood is pumped. The evolution of hematocrit, plasma and extra-fiber fluid osmolality was evaluated without any biological component, to characterize the prototype. LMOs were cut and cold stored 48 h in BG35 and ViaSpan

RESULTS: The concentrations of ammonia and glucose, and the fluids osmolalities were matched after the first hour of perfusion, showing a proper exchange between blood and the biological compartment in the minibioreactor. After 120 min of perfusion, LMOs cold preserved in BG35 and ViaSpan

CONCLUSION: This prototype relied on a simple design and excellent performance. It's a practical tool to evaluate the detoxification ability of LMOs subjected to different preservation protocols.

Keywords: Acute liver failure; BG35 preservation solution; Bioartificial liver device; Cold preservation; Rat liver Microorgans

Conflict of interest statement

Conflict-of-interest statement: No potential conflicts of interest relevant to this article were reported.

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