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Azarpeykan S, Dittmer KE. Evaluation of housekeeping genes for quantitative gene expression analysis in the equine kidney. J Equine Sci. 2016;27(4):165-168doi: 10.1294/jes.27.165.
Azarpeykan, S., & Dittmer, K. E. (2016). Evaluation of housekeeping genes for quantitative gene expression analysis in the equine kidney. Journal of equine science, 27(4), 165-168. https://doi.org/10.1294/jes.27.165
Azarpeykan, Sara, and Dittmer, Keren E. "Evaluation of housekeeping genes for quantitative gene expression analysis in the equine kidney." Journal of equine science vol. 27,4 (2016): 165-168. doi: https://doi.org/10.1294/jes.27.165
Azarpeykan S, Dittmer KE. Evaluation of housekeeping genes for quantitative gene expression analysis in the equine kidney. J Equine Sci. 2016;27(4):165-168. doi: 10.1294/jes.27.165. Epub 2016 Dec 15. PMID: 27974876; PMCID: PMC5155135.
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J Equine Sci. 2016;27(4):165-168. doi: 10.1294/jes.27.165. Epub 2016 Dec 15.

Evaluation of housekeeping genes for quantitative gene expression analysis in the equine kidney.

Journal of equine science

Sara Azarpeykan, Keren E Dittmer

Affiliations

  1. Institute of Veterinary, Animal and Biomedical Science, Massey University, Palmerston North 4442, New Zealand.

PMID: 27974876 PMCID: PMC5155135 DOI: 10.1294/jes.27.165

Abstract

Housekeeping genes (HKGs) are used as internal controls for normalising and calculating the relative expression of target genes in RT-qPCR experiments. There is no unique universal HKG and HKGs vary among organisms and tissues, so this study aimed to determine the most stably expressed HKGs in the equine kidney. The evaluated HKGs included 18S ribosomal RNA (18S), 28S ribosomal RNA (28S), ribosomal protein L32 (RPL32), β-2-microglobulin (B2M), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), succinate dehydrogenase complex (SDHA), zeta polypeptide (YWHAZ), and hypoxanthine phosphoribosyltransferase 1 (HPRT1). The HKGs expression stability data were analysed with two software packages, geNorm and NormFinder. The lowest stability values for geNorm suggests that YWHAZ and HPRT1 would be most optimal (M=0.31 and 0.32, respectively). Further, these two genes had the best pairwise stability value using NormFinder (geNorm V=0.085). Therefore, these two genes were considered the most useful for RT-qPCR studies in equine kidney.

Keywords: horse; housekeeping gene; kidney

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