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Bhat AA, Wani HA, Waza AA, et al. Diminished expression of . J Gastrointest Oncol. 2016;7(6):989-995doi: 10.21037/jgo.2016.06.07.
Bhat, A. A., Wani, H. A., Waza, A. A., Malik, R. A., Masood, A., Jeelani, S., Kadla, S., & Majid, S. (2016). Diminished expression of . Journal of gastrointestinal oncology, 7(6), 989-995. https://doi.org/10.21037/jgo.2016.06.07
Bhat, Arif Akbar, et al. "Diminished expression of ." Journal of gastrointestinal oncology vol. 7,6 (2016): 989-995. doi: https://doi.org/10.21037/jgo.2016.06.07
Bhat AA, Wani HA, Waza AA, Malik RA, Masood A, Jeelani S, Kadla S, Majid S. Diminished expression of . J Gastrointest Oncol. 2016 Dec;7(6):989-995. doi: 10.21037/jgo.2016.06.07. PMID: 28078123; PMCID: PMC5177594.
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J Gastrointest Oncol. 2016 Dec;7(6):989-995. doi: 10.21037/jgo.2016.06.07.

Diminished expression of .

Journal of gastrointestinal oncology

Arif Akbar Bhat, Hilal Ahmad Wani, Ajaz Ahmad Waza, Rawoof Ahmad Malik, Akbar Masood, Showkat Jeelani, Showkat Kadla, Sabhiya Majid

Affiliations

  1. Department of Biochemistry, Government Medical College Srinagar (Research Centre University of Kashmir), Srinagar, India.
  2. Multidisciplinary Research Unit, Government Medical College Srinagar, Srinagar, India.
  3. Department of Biotechnology, University of Kashmir, Hazratbal Srinagar, India.
  4. Department of Human Genetics, Punjabi University Patiala, Punjab, India.
  5. Department of Biochemistry, University of Kashmir, Hazratbal Srinagar, Srinagar, India.

PMID: 28078123 PMCID: PMC5177594 DOI: 10.21037/jgo.2016.06.07

Abstract

BACKGROUND: Cancer initiation and progression are accompanied by profound changes in DNA. DNA methylation that was the first epigenetic alterations identified in cancer. DNA hypermethylation at promoter sites is closely associated with down regulation of protein and as major participant in the development and progression of series of human tumors. Therefore we hypothesized that promoter hypermethylation of

METHODS: A hospital based case-control study; including 200 GC cases and 200 matched controls from patients who went surgical resection. Promoter hypermethylation was determined by Methylation Specific Polymerase chain reaction. The expression of MGMT & RASSF1A protein was examined by Western blotting technique.

RESULTS: Frequency of promoter region hypermethylation of

CONCLUSIONS: These findings indicate that promoter hypermethylation at CpG island may be responsible for reduction of expression at protein level which may be an initial event in carcinogenesis and the progression of GC.

Keywords: CpG island; Methylation specific PCR; epigenetics, hypermethylation; gastric cancer (GC)

Conflict of interest statement

The authors have no conflicts of interest to declare.

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