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Tebianian M, Mosavari N, Taghizadeh M, et al. Evaluation of specific antibodies against Mycobacterium tuberculosis recombinant antigens for detection of recent infection. Int J Mycobacteriol. 2016;5:S254doi: 10.1016/j.ijmyco.2016.11.011.
Tebianian, M., Mosavari, N., Taghizadeh, M., & Ebrahimi, S. M. (2016). Evaluation of specific antibodies against Mycobacterium tuberculosis recombinant antigens for detection of recent infection. International journal of mycobacteriology, 5S254. https://doi.org/10.1016/j.ijmyco.2016.11.011
Tebianian, Majid, et al. "Evaluation of specific antibodies against Mycobacterium tuberculosis recombinant antigens for detection of recent infection." International journal of mycobacteriology vol. 5 (2016): S254. doi: https://doi.org/10.1016/j.ijmyco.2016.11.011
Tebianian M, Mosavari N, Taghizadeh M, Ebrahimi SM. Evaluation of specific antibodies against Mycobacterium tuberculosis recombinant antigens for detection of recent infection. Int J Mycobacteriol. 2016 Dec;5:S254. doi: 10.1016/j.ijmyco.2016.11.011. Epub 2016 Nov 24. PMID: 28043591.
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Int J Mycobacteriol. 2016 Dec;5:S254. doi: 10.1016/j.ijmyco.2016.11.011. Epub 2016 Nov 24.

Evaluation of specific antibodies against Mycobacterium tuberculosis recombinant antigens for detection of recent infection.

International journal of mycobacteriology

Majid Tebianian, Nader Mosavari, Morteza Taghizadeh, Seyyed Mahmoud Ebrahimi

Affiliations

  1. Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran. Electronic address: [email protected].
  2. Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.
  3. Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, P.O. Box 14155-3651, Tehran, Iran.

PMID: 28043591 DOI: 10.1016/j.ijmyco.2016.11.011

Abstract

INTRODUCTION: Since an accurate test for detection of Mycobacterium tuberculosis early infection is urgently needed, this study was designed for development of an efficient screening test in diagnosis of tuberculosis infection.

MATERIALS AND METHODS: In the present study, two recombinant proteins CFP-10, ESAT-6 were tested as antigens for the diagnosis of recent tuberculosis. The proteins were produced in Escherichia coli, purified and tested in indirect ELISAs with sera from 63 subjects with positive clinical results. Also, 56 sera from healthy persons were tested as controls. The results were compared with molecular and culture.

RESULTS: The levels of antibodies against M. tuberculosis antigens in patients with tuberculosis were significantly higher than those in healthy subjects. Among 63 patients, 58 were positive for ESAT-6, 54 for CFP-10.

CONCLUSION: Altogether, the role of M. tuberculosis recombinant proteins, as a suitable candidate for early diagnosis of tuberculosis infection was supported in this study. However, these strongly offer the potential of mixture or fusion of these recombinant proteins for better sensitivity and specificity.

Copyright © 2016.

Keywords: CFP-10; ELISA; ESAT-6; Mycobacterium tuberculosis; Specific antibodies

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