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Danev R, Tegunov D, Baumeister W. Using the Volta phase plate with defocus for cryo-EM single particle analysis. Elife. 2017;6doi: 10.7554/eLife.23006.
Danev, R., Tegunov, D., & Baumeister, W. (2017). Using the Volta phase plate with defocus for cryo-EM single particle analysis. eLife, 6. https://doi.org/10.7554/eLife.23006
Danev, Radostin, et al. "Using the Volta phase plate with defocus for cryo-EM single particle analysis." eLife vol. 6 (2017). doi: https://doi.org/10.7554/eLife.23006
Danev R, Tegunov D, Baumeister W. Using the Volta phase plate with defocus for cryo-EM single particle analysis. Elife. 2017 Jan 21;6. doi: 10.7554/eLife.23006. PMID: 28109158; PMCID: PMC5279940.
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Elife. 2017 Jan 21;6. doi: 10.7554/eLife.23006.

Using the Volta phase plate with defocus for cryo-EM single particle analysis.

eLife

Radostin Danev, Dimitry Tegunov, Wolfgang Baumeister

Affiliations

  1. Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.
  2. Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.

PMID: 28109158 PMCID: PMC5279940 DOI: 10.7554/eLife.23006

Abstract

Previously, we reported an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate (Danev and Baumeister, 2016). Here, we extend the technique to include a small amount of defocus which enables contrast transfer function measurement and correction. This hybrid approach simplifies the experiment and increases the data acquisition speed. It also removes the resolution limit inherent to the in-focus method thus allowing 3D reconstructions with resolutions better than 3 Å.

Keywords: biophysics; cryo-EM; none; phase plate; proteasome; structural biology

Conflict of interest statement

RD: RD is a co-inventor in US patent US9129774 B2 "Method of using a phase plate in a transmission electron microscope". WB: WB is on the Scientific Advisory Board of FEI Company. The other author dec

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