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Chan JMS, Cheung MSH, Gibbs RGJ, et al. MRI detection of endothelial cell inflammation using targeted superparamagnetic particles of iron oxide (SPIO). Clin Transl Med. 2017;6(1):1doi: 10.1186/s40169-016-0134-1.
Chan, J. M. S., Cheung, M. S. H., Gibbs, R. G. J., & Bhakoo, K. K. (2017). MRI detection of endothelial cell inflammation using targeted superparamagnetic particles of iron oxide (SPIO). Clinical and translational medicine, 6(1), 1. https://doi.org/10.1186/s40169-016-0134-1
Chan, Joyce M S, et al. "MRI detection of endothelial cell inflammation using targeted superparamagnetic particles of iron oxide (SPIO)." Clinical and translational medicine vol. 6,1 (2017): 1. doi: https://doi.org/10.1186/s40169-016-0134-1
Chan JMS, Cheung MSH, Gibbs RGJ, Bhakoo KK. MRI detection of endothelial cell inflammation using targeted superparamagnetic particles of iron oxide (SPIO). Clin Transl Med. 2017 Dec;6(1):1. doi: 10.1186/s40169-016-0134-1. Epub 2017 Jan 02. PMID: 28044245; PMCID: PMC5206220.
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Clin Transl Med. 2017 Dec;6(1):1. doi: 10.1186/s40169-016-0134-1. Epub 2017 Jan 02.

MRI detection of endothelial cell inflammation using targeted superparamagnetic particles of iron oxide (SPIO).

Clinical and translational medicine

Joyce M S Chan, Maggie S H Cheung, Richard G J Gibbs, Kishore K Bhakoo

Affiliations

  1. Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong SAR, People's Republic of China. [email protected].
  2. Regional Vascular Unit, St Mary's Hospital, Imperial College Healthcare NHS Trust, Imperial College London, London, UK. [email protected].
  3. Translational Molecular Imaging Group, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), 11 Biopolis Way, Helios, 138667, Singapore. [email protected].
  4. Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong SAR, People's Republic of China.
  5. Regional Vascular Unit, St Mary's Hospital, Imperial College Healthcare NHS Trust, Imperial College London, London, UK.
  6. Translational Molecular Imaging Group, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), 11 Biopolis Way, Helios, 138667, Singapore.

PMID: 28044245 PMCID: PMC5206220 DOI: 10.1186/s40169-016-0134-1

Abstract

BACKGROUND: There is currently no clinical imaging technique available to assess the degree of inflammation associated with atherosclerotic plaques. This study aims to develop targeted superparamagnetic particles of iron oxide (SPIO) as a magnetic resonance imaging (MRI) probe for detecting inflamed endothelial cells.

METHODS: The in vitro study consists of the characterisation and detection of inflammatory markers on activated endothelial cells by immunocytochemistry and MRI using biotinylated anti-P-selectin and anti-VCAM-1 (vascular cell adhesion molecule 1) antibody and streptavidin conjugated SPIO.

RESULTS: Established an in vitro cellular model of endothelial inflammation induced with TNF-α (tumor necrosis factor alpha). Inflammation of endothelial cells was confirmed with both immunocytochemistry and MRI. These results revealed both a temporal and dose dependent expression of the inflammatory markers, P-selectin and VCAM-1, on exposure to TNF-α.

CONCLUSION: This study has demonstrated the development of an in vitro model to characterise and detect inflamed endothelial cells by immunocytochemistry and MRI. This will allow the future development of contrast agents and protocols for imaging vascular inflammation in atherosclerosis. This work may form the basis for a translational study to provide clinicians with a novel tool for the in vivo assessment of atherosclerosis.

Keywords: Atherosclerosis; Endothelial adhesion molecules; Inflammation imaging; MRI; Superparamagnetic particles of iron oxide; Vascular imaging

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