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Salerno M, Shayganpour A, Salis B, et al. Surface-enhanced Raman scattering of self-assembled thiol monolayers and supported lipid membranes on thin anodic porous alumina. Beilstein J Nanotechnol. 2017;8:74-81doi: 10.3762/bjnano.8.8.
Salerno, M., Shayganpour, A., Salis, B., & Dante, S. (2017). Surface-enhanced Raman scattering of self-assembled thiol monolayers and supported lipid membranes on thin anodic porous alumina. Beilstein journal of nanotechnology, 874-81. https://doi.org/10.3762/bjnano.8.8
Salerno, Marco, et al. "Surface-enhanced Raman scattering of self-assembled thiol monolayers and supported lipid membranes on thin anodic porous alumina." Beilstein journal of nanotechnology vol. 8 (2017): 74-81. doi: https://doi.org/10.3762/bjnano.8.8
Salerno M, Shayganpour A, Salis B, Dante S. Surface-enhanced Raman scattering of self-assembled thiol monolayers and supported lipid membranes on thin anodic porous alumina. Beilstein J Nanotechnol. 2017 Jan 09;8:74-81. doi: 10.3762/bjnano.8.8. eCollection 2017. PMID: 28144566; PMCID: PMC5238693.
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Beilstein J Nanotechnol. 2017 Jan 09;8:74-81. doi: 10.3762/bjnano.8.8. eCollection 2017.

Surface-enhanced Raman scattering of self-assembled thiol monolayers and supported lipid membranes on thin anodic porous alumina.

Beilstein journal of nanotechnology

Marco Salerno, Amirreza Shayganpour, Barbara Salis, Silvia Dante

Affiliations

  1. Department of Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, I-16163 Genova, Italy.
  2. Department of Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, I-16163 Genova, Italy; Department of Bioengineering and Robotics, University of Genova, viale Causa 13, I-16145 Genova, Italy.

PMID: 28144566 PMCID: PMC5238693 DOI: 10.3762/bjnano.8.8

Abstract

Thin anodic porous alumina (tAPA) was fabricated from a 500 nm thick aluminum (Al) layer coated on silicon wafers, through single-step anodization performed in a Teflon electrochemical cell in 0.4 M aqueous phosphoric acid at 110 V. Post-fabrication etching in the same acid allowed obtaining tAPA surfaces with ≈160 nm pore diameter and ≈80 nm corresponding wall thickness to be prepared. The tAPA surfaces were made SERS-active by coating with a thin (≈25 nm) gold (Au) layer. The as obtained tAPA-Au substrates were incubated first with different thiols, namely mercaptobenzoic acid (MbA) and aminothiol (AT), and then with phospholipid vesicles of different composition to form a supported lipid bilayer (SLB). At each step, the SERS substrate functionality was assessed, demonstrating acceptable enhancement (≥100×). The chemisorption of thiols during the first step and the formation of SLB from the vesicles during the second step, were independently monitored by using a quartz crystal microbalance with dissipation monitoring (QCM-D) technique. The SLB membranes represent a simplified model system of the living cells membranes, which makes the successful observation of SERS on these films promising in view of the use of tAPA-Au substrates as a platform for the development of surface-enhanced Raman spectroscopy (SERS) biosensors on living cells. In the future, these tAPA-Au-SLB substrates will be investigated also for drug delivery of bioactive agents from the APA pores.

Keywords: SERS; anodic porous alumina; nanopores; supported lipid bilayers; thiols

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