Display options
Cite
Damle SG, Loomba A, Dhindsa A, et al. Correlation between dental caries experience and mutans streptococci counts by microbial and molecular (polymerase chain reaction) assay using saliva as microbial risk indicator. Dent Res J (Isfahan). 2016;13(6):552-559doi: 10.4103/1735-3327.197035.
Damle, S. G., Loomba, A., Dhindsa, A., Loomba, A., & Beniwal, V. (2016). Correlation between dental caries experience and mutans streptococci counts by microbial and molecular (polymerase chain reaction) assay using saliva as microbial risk indicator. Dental research journal, 13(6), 552-559. https://doi.org/10.4103/1735-3327.197035
Damle, S G, et al. "Correlation between dental caries experience and mutans streptococci counts by microbial and molecular (polymerase chain reaction) assay using saliva as microbial risk indicator." Dental research journal vol. 13,6 (2016): 552-559. doi: https://doi.org/10.4103/1735-3327.197035
Damle SG, Loomba A, Dhindsa A, Loomba A, Beniwal V. Correlation between dental caries experience and mutans streptococci counts by microbial and molecular (polymerase chain reaction) assay using saliva as microbial risk indicator. Dent Res J (Isfahan). 2016 Nov-Dec;13(6):552-559. doi: 10.4103/1735-3327.197035. PMID: 28182053; PMCID: PMC5256021.
Copy Download .nbib Format: NLM
Share it on

Dent Res J (Isfahan). 2016 Nov-Dec;13(6):552-559. doi: 10.4103/1735-3327.197035.

Correlation between dental caries experience and mutans streptococci counts by microbial and molecular (polymerase chain reaction) assay using saliva as microbial risk indicator.

Dental research journal

S G Damle, Ashish Loomba, Abhishek Dhindsa, Ashu Loomba, Vikas Beniwal

Affiliations

  1. Department of Pediatric and Preventive Dentistry, M.M. College of Dental Sciences and Research, M.M. University, Mullana, Haryana, India.
  2. Department of Public Health Dentistry, Dr. Loomba's Family Dental Clinic, Mohali, Punjab, India.
  3. Department of Biotechnology, M.M. Engineering College, M.M. University, Mullana, Haryana, India.

PMID: 28182053 PMCID: PMC5256021 DOI: 10.4103/1735-3327.197035

Abstract

BACKGROUND: The aim of this study was to assess the relationships of quantitative salivary levels of mutans streptococci (MS) in children, aged 3-6 years and 12-15 years, exhibiting variable patterns of caries activity, and to compare the association of MS in saliva using microbial and molecular (polymerase chain reaction [PCR]) assay.

MATERIALS AND METHODS: In this cross-sectional observational study, eighty children were included, forty children each in Group I (3-6 years) and Group II (12-15 years). Children were further divided into two subgroups (Group IC, INC and Group IIC, IINC) based on their dental caries status. Saliva samples were collected and plated onto Mitis Salivarius-Bacitracin agar plates. After detection of MS, DNA was isolated and purified, and MS were evaluated using the PCR and AP-PCR.

RESULTS: Of the selected 80 children, 42 were male and 38 were female. In Group IC, the mean colony count was 2.27 ± 0.54 × 10

CONCLUSION: The mean colony count increased with increasing age and was also more in children with dental caries. Based on the matrices generated by the PCR analysis using coefficient slipped-strand mispairing, wide range of genetic diversity was seen in cases of children with and without clinically detectable caries.

Keywords: Dental caries; polymerase chain reaction; saliva

Conflict of interest statement

The authors of this manuscript declare that they have no conflicts of interest, real or perceived, financial or non-financial in this article.

References

  1. Int J Syst Bacteriol. 1995 Apr;45(2):406-8 - PubMed
  2. J Med Microbiol. 1995 Jan;42(1):67-72 - PubMed
  3. Oral Microbiol Immunol. 1997 Oct;12(5):266-73 - PubMed
  4. J Oral Sci. 2006 Dec;48(4):233-7 - PubMed
  5. Caries Res. 2004 May-Jun;38(3):182-91 - PubMed
  6. Clin Oral Investig. 2015 Nov;19(8):1955-64 - PubMed
  7. Braz Dent J. 2007;18(4):329-33 - PubMed
  8. J Clin Pediatr Dent. 2016;40(4):281-9 - PubMed
  9. Community Dent Oral Epidemiol. 1982 Apr;10(2):77-81 - PubMed
  10. J Oral Microbiol. 2015 Apr 01;7:27182 - PubMed
  11. Arch Oral Biol. 2007 Sep;52(9):876-81 - PubMed
  12. Rev Med Chir Soc Med Nat Iasi. 2015 Oct-Dec;119(4):996-1000 - PubMed
  13. J Microbiol Methods. 2001 Aug;46(2):99-105 - PubMed
  14. J Med Microbiol. 1996 Oct;45(4):278-84 - PubMed
  15. Oral Microbiol Immunol. 1990 Jun;5(3):169-71 - PubMed
  16. Microbiology. 2003 Feb;149(Pt 2):279-94 - PubMed
  17. J Dent Res. 2003 Jul;82(7):504-8 - PubMed
  18. J Clin Microbiol. 2008 Apr;46(4):1407-17 - PubMed
  19. J Med Microbiol. 2004 Jul;53(Pt 7):697-703 - PubMed
  20. J Clin Pediatr Dent. 2008 Winter;32(2):121-6 - PubMed
  21. J Med Microbiol. 1981 Feb;14(1):51-62 - PubMed
  22. J Indian Soc Pedod Prev Dent. 2005 Mar;23(1):23-6 - PubMed
  23. Arch Oral Biol. 2001 May;46(5):471-6 - PubMed
  24. PLoS One. 2015 May 28;10(5):e0128534 - PubMed
  25. J Clin Exp Dent. 2015 Feb 01;7(1):e114-8 - PubMed
  26. ASDC J Dent Child. 1997 Mar-Apr;64(2):107-11 - PubMed
  27. Mol Cell Probes. 1994 Feb;8(1):73-80 - PubMed
  28. Int J Dent. 2010;2010:824976 - PubMed
  29. Oral Microbiol Immunol. 2006 Feb;21(1):47-52 - PubMed
  30. Oral Microbiol Immunol. 2007 Oct;22(5):313-9 - PubMed

Publication Types