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Barretto SS, Michoux F, Hellgardt K, et al. Pneumatic hydrodynamics influence transplastomic protein yields and biological responses during . Biochem Eng J. 2017;117:73-81doi: 10.1016/j.bej.2016.10.007.
Barretto, S. S., Michoux, F., Hellgardt, K., & Nixon, P. J. (2017). Pneumatic hydrodynamics influence transplastomic protein yields and biological responses during . Biochemical engineering journal, 11773-81. https://doi.org/10.1016/j.bej.2016.10.007
Barretto, Sherwin S, et al. "Pneumatic hydrodynamics influence transplastomic protein yields and biological responses during ." Biochemical engineering journal vol. 117 (2017): 73-81. doi: https://doi.org/10.1016/j.bej.2016.10.007
Barretto SS, Michoux F, Hellgardt K, Nixon PJ. Pneumatic hydrodynamics influence transplastomic protein yields and biological responses during . Biochem Eng J. 2017 Jan 15;117:73-81. doi: 10.1016/j.bej.2016.10.007. PMID: 28111521; PMCID: PMC5221668.
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Biochem Eng J. 2017 Jan 15;117:73-81. doi: 10.1016/j.bej.2016.10.007.

Pneumatic hydrodynamics influence transplastomic protein yields and biological responses during .

Biochemical engineering journal

Sherwin S Barretto, Franck Michoux, Klaus Hellgardt, Peter J Nixon

Affiliations

  1. Department of Life Sciences, Imperial College London, South Kensington, London SW7 2AZ, United Kingdom.
  2. Alkion Biopharma SAS, Pépinière Entreprise Genopole, 4 rue Pierre Fontaine, 91058, Evry, France.
  3. Department of Chemical Engineering, Imperial College London, South Kensington, London SW7 2AZ, United Kingdom.

PMID: 28111521 PMCID: PMC5221668 DOI: 10.1016/j.bej.2016.10.007

Abstract

Transplastomic plants are capable of high-yield production of recombinant biopharmaceutical proteins. Plant tissue culture combines advantages of agricultural cultivation with the bioprocess consistency associated with suspension culture. Overexpression of recombinant proteins through regeneration of transplastomic

Keywords: Biopharmaceutical; CIM, callus induction medium; Hydrodynamics; MS medium, Murashige & Skoog medium; Pneumatic energy dissipation; RITA®, recipient for automated temporary immersion (translated from French); SDS-PAGE, sodium dodecyl sulphate polyacrylamide gel electrophoresis; TF, triphenylformazan; TIB, temporary immersion bioreactor; TSP, total soluble protein; TTC, 2,3,5-triphenyltetrazolium chloride; Temporary immersion culture; TetC, fragment C of tetanus toxin; Transplastomic protein; in vitro organogenesis; kDa, kiloDalton

References

  1. Biotechnol Bioeng. 1998 Jun 20;58(6):595-604 - PubMed
  2. Biotechnol Bioeng. 2000 Mar 20;67(6):775-90 - PubMed
  3. Adv Biochem Eng Biotechnol. 2000;67:139-77 - PubMed
  4. Tree Physiol. 2003 Mar;23(4):257-63 - PubMed
  5. Nucleic Acids Res. 2003 Feb 15;31(4):1174-9 - PubMed
  6. Appl Biochem Biotechnol. 2003 Aug;110(2):61-74 - PubMed
  7. Phytochemistry. 2004 Apr;65(8):989-94 - PubMed
  8. Planta. 2006 Jun;224(1):72-82 - PubMed
  9. Transgenic Res. 2006 Apr;15(2):205-17 - PubMed
  10. Biotechnol Lett. 2006 Mar;28(6):431-8 - PubMed
  11. Nucleic Acids Res. 1991 Mar 11;19(5):983-95 - PubMed
  12. Proc Natl Acad Sci U S A. 2007 Apr 24;104(17):6998-7002 - PubMed
  13. Biotechnol Bioeng. 1982 Feb;24(2):425-42 - PubMed
  14. J Neurosci Methods. 2008 Jul 30;172(2):250-4 - PubMed
  15. Biotechnol Bioeng. 1989 Dec 20;34(11):1391-2 - PubMed
  16. Biotechnol Appl Biochem. 2009 Jun 22;53(Pt 4):265-75 - PubMed
  17. Biotechnol Adv. 2009 Nov-Dec;27(6):1036-42 - PubMed
  18. Appl Microbiol Biotechnol. 2009 Jul;83(5):809-23 - PubMed
  19. Biotechnol Adv. 2009 Nov-Dec;27(6):879-94 - PubMed
  20. Biotechnol Bioeng. 2010 Feb 15;105(3):588-99 - PubMed
  21. Plant Biotechnol J. 2011 Jun;9(5):575-84 - PubMed
  22. Biotechnol Adv. 2011 May-Jun;29(3):278-99 - PubMed
  23. Biotechnol Adv. 2012 Sep-Oct;30(5):1171-84 - PubMed
  24. Planta. 2013 Mar;237(3):903-8 - PubMed
  25. Curr Pharm Des. 2013;19(31):5486-94 - PubMed
  26. Curr Opin Biotechnol. 2014 Apr;26:7-13 - PubMed
  27. Biotechnol Lett. 2014 Dec;36(12):2367-79 - PubMed
  28. Methods Mol Biol. 2016;1385:149-60 - PubMed
  29. Anal Biochem. 1976 May 7;72:248-54 - PubMed
  30. J Biotechnol. 1997 Dec 17;59(1-2):39-52 - PubMed
  31. Biotechnol Prog. 1998 Jul;14(4):607-14 - PubMed

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