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Bhatt P, Tandel K, Singh A, et al. Prevalence and molecular characterization of methicillin resistance among Coagulase-negative Staphylococci at a tertiary care center. Med J Armed Forces India. 2016;72:S54-S58doi: 10.1016/j.mjafi.2016.03.007.
Bhatt, P., Tandel, K., Singh, A., Kumar, M., Grover, N., & Sahni, A. K. (2016). Prevalence and molecular characterization of methicillin resistance among Coagulase-negative Staphylococci at a tertiary care center. Medical journal, Armed Forces India, 72S54-S58. https://doi.org/10.1016/j.mjafi.2016.03.007
Bhatt, Puneet, et al. "Prevalence and molecular characterization of methicillin resistance among Coagulase-negative Staphylococci at a tertiary care center." Medical journal, Armed Forces India vol. 72 (2016): S54-S58. doi: https://doi.org/10.1016/j.mjafi.2016.03.007
Bhatt P, Tandel K, Singh A, Kumar M, Grover N, Sahni AK. Prevalence and molecular characterization of methicillin resistance among Coagulase-negative Staphylococci at a tertiary care center. Med J Armed Forces India. 2016 Dec;72:S54-S58. doi: 10.1016/j.mjafi.2016.03.007. Epub 2016 Jun 24. PMID: 28050071; PMCID: PMC5192199.
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Med J Armed Forces India. 2016 Dec;72:S54-S58. doi: 10.1016/j.mjafi.2016.03.007. Epub 2016 Jun 24.

Prevalence and molecular characterization of methicillin resistance among Coagulase-negative Staphylococci at a tertiary care center.

Medical journal, Armed Forces India

Puneet Bhatt, Kundan Tandel, Alina Singh, M Kumar, Naveen Grover, A K Sahni

Affiliations

  1. Graded Specialist (Microbiology), Command Hospital (Southern Command), Pune 411040, India.
  2. Graded Specialist (Microbiology), Defence Research & Development Establishment, Gwalior, India.
  3. Assistant Professor, Nepalese Army Institute of Health Sciences, Nepal.
  4. Professor, Dept of Microbiology, Armed Forces Medical College, Pune 411040, India.
  5. Professor & Head, Dept of Microbiology, Armed Forces Medical College, Pune 411040, India.
  6. Commandant, 174 Military Hospital, C/o 56 APO, India.

PMID: 28050071 PMCID: PMC5192199 DOI: 10.1016/j.mjafi.2016.03.007

Abstract

BACKGROUND: Methicillin-resistant Coagulase-negative Staphylococci (MR-CoNS) have emerged as an important cause of nosocomial infections especially in patients with prosthetic devices and implants. This study was conducted with an aim to determine the prevalence of methicillin resistance among CoNS isolates at a tertiary care center by both phenotypic and genotypic methods.

METHODS: This cross sectional study was carried out from September 2011 to February 2014 in which 150 non-repetitive clinical isolates of CoNS were identified at the species level by conventional phenotypic methods. Cefoxitin disk (30 μg) diffusion testing was used to determine methicillin resistance and confirmed by detection of

RESULTS: Out of 150 CoNS isolates, 51 were methicillin resistant by cefoxitin disk diffusion method. Out of these 51 isolates,

CONCLUSION: The prevalence of methicillin resistance among Coagulase-negative Staphylococci (CoNS) was 32.7% by PCR detection of

Keywords: Cefoxitin disk; Methicillin-resistant Coagulase-negative Staphylococci (MR-CoNS); Polymerase chain reaction (PCR); mecA gene

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