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Lisov AV, Belova OV, Lisova ZA, et al. Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential. AMB Express. 2017;7(1):5doi: 10.1186/s13568-016-0308-7.
Lisov, A. V., Belova, O. V., Lisova, Z. A., Vinokurova, N. G., Nagel, A. S., Andreeva-Kovalevskaya, Z. I., Budarina, Z. I., Nagornykh, M. O., Zakharova, M. V., Shadrin, A. M., Solonin, A. S., & Leontievsky, A. A. (2017). Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential. AMB Express, 7(1), 5. https://doi.org/10.1186/s13568-016-0308-7
Lisov, Alexander V, et al. "Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential." AMB Express vol. 7,1 (2017): 5. doi: https://doi.org/10.1186/s13568-016-0308-7
Lisov AV, Belova OV, Lisova ZA, Vinokurova NG, Nagel AS, Andreeva-Kovalevskaya ZI, Budarina ZI, Nagornykh MO, Zakharova MV, Shadrin AM, Solonin AS, Leontievsky AA. Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential. AMB Express. 2017 Dec;7(1):5. doi: 10.1186/s13568-016-0308-7. Epub 2017 Jan 03. PMID: 28050845; PMCID: PMC5209306.
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AMB Express. 2017 Dec;7(1):5. doi: 10.1186/s13568-016-0308-7. Epub 2017 Jan 03.

Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential.

AMB Express

Alexander V Lisov, Oksana V Belova, Zoya A Lisova, Nataliy G Vinokurova, Alexey S Nagel, Zhanna I Andreeva-Kovalevskaya, Zhanna I Budarina, Maxim O Nagornykh, Marina V Zakharova, Andrey M Shadrin, Alexander S Solonin, Alexey A Leontievsky

Affiliations

  1. G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia. [email protected].
  2. G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
  3. Pushchino State Institute of Life Sciences, 3 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.

PMID: 28050845 PMCID: PMC5209306 DOI: 10.1186/s13568-016-0308-7

Abstract

Four xylanases of Cellulomonas flavigena were cloned, expressed in Escherichia coli and purified. Three enzymes (CFXyl1, CFXyl2, and CFXyl4) were from the GH10 family, while CFXyl3 was from the GH11 family. The enzymes possessed moderate temperature stability and a neutral pH optimum. The enzymes were more stable at alkaline pH values. CFXyl1 and CFXyl2 hydrolyzed xylan to form xylobiose, xylotriose, xylohexaose, xylopentaose, and xylose, which is typical for GH10. CFXyl3 (GH11) and CFXyl4 (GH10) formed the same xylooligosaccharides, but xylose was formed in small amounts. The xylanases made efficient saccharification of rye, wheat and oat, common components of animal feed, which indicates their high biotechnological potential.

Keywords: Cellulomonas flavigena; Saccharification of cereals; Xylanases; Xylooligosaccharides

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