Display options
Cite
Deka SJ, Mamdi N, Manna D, et al. Alkyl Cinnamates Induce Protein Kinase C Translocation and Anticancer Activity against Breast Cancer Cells through Induction of the Mitochondrial Pathway of Apoptosis. J Breast Cancer. 2016;19(4):358-371doi: 10.4048/jbc.2016.19.4.358.
Deka, S. J., Mamdi, N., Manna, D., & Trivedi, V. (2016). Alkyl Cinnamates Induce Protein Kinase C Translocation and Anticancer Activity against Breast Cancer Cells through Induction of the Mitochondrial Pathway of Apoptosis. Journal of breast cancer, 19(4), 358-371. https://doi.org/10.4048/jbc.2016.19.4.358
Deka, Suman Jyoti, et al. "Alkyl Cinnamates Induce Protein Kinase C Translocation and Anticancer Activity against Breast Cancer Cells through Induction of the Mitochondrial Pathway of Apoptosis." Journal of breast cancer vol. 19,4 (2016): 358-371. doi: https://doi.org/10.4048/jbc.2016.19.4.358
Deka SJ, Mamdi N, Manna D, Trivedi V. Alkyl Cinnamates Induce Protein Kinase C Translocation and Anticancer Activity against Breast Cancer Cells through Induction of the Mitochondrial Pathway of Apoptosis. J Breast Cancer. 2016 Dec;19(4):358-371. doi: 10.4048/jbc.2016.19.4.358. Epub 2016 Dec 23. PMID: 28053624; PMCID: PMC5204042.
Copy Download .nbib Format: NLM
Share it on

J Breast Cancer. 2016 Dec;19(4):358-371. doi: 10.4048/jbc.2016.19.4.358. Epub 2016 Dec 23.

Alkyl Cinnamates Induce Protein Kinase C Translocation and Anticancer Activity against Breast Cancer Cells through Induction of the Mitochondrial Pathway of Apoptosis.

Journal of breast cancer

Suman Jyoti Deka, Narsimha Mamdi, Debasis Manna, Vishal Trivedi

Affiliations

  1. Malaria Research Group, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, India.
  2. Laboratory of Biological Chemistry, Department of Chemistry, Indian Institute of Technology Guwahati, Guwahati, India.

PMID: 28053624 PMCID: PMC5204042 DOI: 10.4048/jbc.2016.19.4.358

Abstract

PURPOSE: The protein kinase C (PKC) family of serine-threonine kinases plays an important role in cancer cell progression. Thus, molecules that target PKC have potential as anticancer agents. The current study aims to understand the treatment of breast cancer cells with alkyl cinnamates. We have also explored the mechanistic details of their anticancer action and the underlying molecular signaling.

METHODS: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to measure the viability of MDAMB-231 breast cancer cells to assess the anticancer activity of these compounds. In addition, flow cytometry was performed to study the effect of alkyl cinnamates on the cell cycle and apoptosis. Immunoblotting and immunofluorescence techniques were performed to study PKC translocation, cytochrome c release, and modulation of the mitochondrial membrane potential in breast cancer cells targeted with alkyl cinnamates.

RESULTS: The PKC agonist DM-2-8 translocated 16.6%±1.7% PKCα from cytosol to the plasma membrane and showed excellent anticancer activity with an half maximal inhibitory concentration (IC

CONCLUSION: Alkyl cinnamates specifically target cancer cells through induction of PKC translocation and the mitochondrial pathway of apoptosis, and could be promising anticancer drugs.

Keywords: Apoptosis; Caspases; Neoplasms; Oxidative stress; Protein kinase C

Conflict of interest statement

The authors declare that they have no competing interests.

References

  1. PLoS One. 2014 Jul 31;9(7):e103706 - PubMed
  2. Bioorg Med Chem Lett. 2009 Dec 1;19(23):6627-31 - PubMed
  3. Proc Natl Acad Sci U S A. 2006 Nov 28;103(48):18226-31 - PubMed
  4. FEBS Lett. 1999 Feb 19;445(1):165-8 - PubMed
  5. Biochem Biophys Res Commun. 1999 Jul 14;260(3):682-5 - PubMed
  6. J Biol Chem. 1997 Apr 4;272(14):9424-35 - PubMed
  7. Asian Pac J Cancer Prev. 2014;15(9):3987-92 - PubMed
  8. Cell. 2000 Jan 7;100(1):57-70 - PubMed
  9. Cancer Res. 1989 Jun 15;49(12):3215-7 - PubMed
  10. Chem Phys Lipids. 2012 Apr;165(3):320-30 - PubMed
  11. Cancer Lett. 2006 Apr 8;235(1):1-10 - PubMed
  12. Cell Mol Life Sci. 2003 Jun;60(6):1061-70 - PubMed
  13. J Biol Chem. 2005 Sep 16;280(37):32107-14 - PubMed
  14. Front Immunol. 2013 Jan 17;3:423 - PubMed
  15. J Cell Biochem. 2008 Jan 1;103(1):9-20 - PubMed
  16. Eur J Pharmacol. 1998 Oct 30;360(1):65-71 - PubMed
  17. J Biol Chem. 2005 Dec 16;280(50):41129-36 - PubMed
  18. J Biol Chem. 2004 Mar 5;279(10):9233-47 - PubMed
  19. J Biol Chem. 2003 Sep 5;278(36):33753-62 - PubMed
  20. J Invest Dermatol. 2012 Aug;132(8):1988-97 - PubMed
  21. Biochem Pharmacol. 1989 May 15;38(10):1617-24 - PubMed
  22. Nat Rev Cancer. 2007 Apr;7(4):281-94 - PubMed
  23. J Exp Clin Cancer Res. 2011 Sep 26;30:87 - PubMed
  24. Cancer Res. 2008 Mar 15;68(6):1962-9 - PubMed
  25. J Biol Chem. 2002 Jan 4;277(1):645-55 - PubMed
  26. Zhonghua Zhong Liu Za Zhi. 2006 Aug;28(8):564-7 - PubMed
  27. Proc Natl Acad Sci U S A. 2011 Apr 26;108(17):6721-6 - PubMed
  28. Cell Growth Differ. 1996 Apr;7(4):419-28 - PubMed
  29. Anticancer Drugs. 2006 Mar;17(3):279-87 - PubMed

Publication Types