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Saluzzo J, Hallman KM, Aleck K, et al. The regulation of tumor suppressor protein, p53, and estrogen receptor (ERα) by resveratrol in breast cancer cells. Genes Cancer. 2016;7(11):414-425doi: 10.18632/genesandcancer.125.
Saluzzo, J., Hallman, K. M., Aleck, K., Dwyer, B., Quigley, M., Mladenovik, V., Siebert, A. E., & Dinda, S. (2016). The regulation of tumor suppressor protein, p53, and estrogen receptor (ERα) by resveratrol in breast cancer cells. Genes & cancer, 7(11), 414-425. https://doi.org/10.18632/genesandcancer.125
Saluzzo, Julieta, et al. "The regulation of tumor suppressor protein, p53, and estrogen receptor (ERα) by resveratrol in breast cancer cells." Genes & cancer vol. 7,11 (2016): 414-425. doi: https://doi.org/10.18632/genesandcancer.125
Saluzzo J, Hallman KM, Aleck K, Dwyer B, Quigley M, Mladenovik V, Siebert AE, Dinda S. The regulation of tumor suppressor protein, p53, and estrogen receptor (ERα) by resveratrol in breast cancer cells. Genes Cancer. 2016 Nov;7(11):414-425. doi: 10.18632/genesandcancer.125. PMID: 28191286; PMCID: PMC5302041.
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Genes Cancer. 2016 Nov;7(11):414-425. doi: 10.18632/genesandcancer.125.

The regulation of tumor suppressor protein, p53, and estrogen receptor (ERα) by resveratrol in breast cancer cells.

Genes & cancer

Julieta Saluzzo, Kelly M Hallman, Katie Aleck, Brigitte Dwyer, Meghan Quigley, Viktoria Mladenovik, Amy E Siebert, Sumi Dinda

Affiliations

  1. School of Health Sciences, Oakland University, Rochester, MI, USA; Prevention Research Center, Oakland University, Rochester, MI, USA.

PMID: 28191286 PMCID: PMC5302041 DOI: 10.18632/genesandcancer.125

Abstract

Resveratrol (RES) is a natural antioxidant found abundantly in grapes, peanuts, and berries, and is known to possess anti-tumorigenic properties. However, there is a noticeable lack of studies on the mechanistic effects of Resveratrol on tumor suppressors. Previous studies from our laboratory have shown the tumor suppressor protein p53 and estrogen receptor-alpha (ERα) to be possible molecular targets for RES. In this study, the anti-estrogenic effects of RES were analyzed on the expression of ERα and p53. The breast cancer cells grown in stripped serum were treated with 60 μM RES, as the optimum concentration based on data obtained from a concentration study using 1-100 μM RES. Our studies indicate that RES caused a decrease in the levels of protein expression of p53 and ERα as compared to the control. Increasing concentrations of RES caused a four-fold decrease in cell number in comparison to estradiol. RES, in conjunction with ICI 182,780 (ICI), caused a down-regulation of both p53 and ERα as compared to the control. These observed effects on cell proliferation and regulation of both p53 and ERα by RES may lead to further understanding of the relationship between tumor suppressor proteins and steroid receptors in breast cancer cells.

Keywords: Breast cancer; antiestrogens; estrogen; p53; tumor suppressors

Conflict of interest statement

None

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