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Malheiro V, Elbs-Glatz Y, Obarzanek-Fojt M, et al. Harvesting pre-polarized macrophages using thermo-responsive substrates. Sci Rep. 2017;7:42495doi: 10.1038/srep42495.
Malheiro, V., Elbs-Glatz, Y., Obarzanek-Fojt, M., Maniura-Weber, K., & Bruinink, A. (2017). Harvesting pre-polarized macrophages using thermo-responsive substrates. Scientific reports, 742495. https://doi.org/10.1038/srep42495
Malheiro, Vera, et al. "Harvesting pre-polarized macrophages using thermo-responsive substrates." Scientific reports vol. 7 (2017): 42495. doi: https://doi.org/10.1038/srep42495
Malheiro V, Elbs-Glatz Y, Obarzanek-Fojt M, Maniura-Weber K, Bruinink A. Harvesting pre-polarized macrophages using thermo-responsive substrates. Sci Rep. 2017 Feb 14;7:42495. doi: 10.1038/srep42495. PMID: 28195152; PMCID: PMC5307341.
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Sci Rep. 2017 Feb 14;7:42495. doi: 10.1038/srep42495.

Harvesting pre-polarized macrophages using thermo-responsive substrates.

Scientific reports

Vera Malheiro, Yvonne Elbs-Glatz, Magdalena Obarzanek-Fojt, Katharina Maniura-Weber, Arie Bruinink

Affiliations

  1. Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstr. 5, CH-9014 St. Gallen, Switzerland.

PMID: 28195152 PMCID: PMC5307341 DOI: 10.1038/srep42495

Abstract

In the cell culture environment macrophages are highly adherent cells. Currently used methods to harvest macrophages have the disadvantage of reducing cell viability and their ability to re-attach after seeding. Although thermo-responsive surfaces have been employed to harvest cell sheets no reports are available to use these to harvest (pre-polarized) macrophages. We show that this method significantly improves the yield of living macrophages and percentage of subsequent cell reattachment, whilst having a minimal effect on the cell phenotype.

Conflict of interest statement

The authors declare no competing financial interests.

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