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Menke S, Gillingham MA, Wilhelm K, et al. Home-Made Cost Effective Preservation Buffer Is a Better Alternative to Commercial Preservation Methods for Microbiome Research. Front Microbiol. 2017;8:102doi: 10.3389/fmicb.2017.00102.
Menke, S., Gillingham, M. A., Wilhelm, K., & Sommer, S. (2017). Home-Made Cost Effective Preservation Buffer Is a Better Alternative to Commercial Preservation Methods for Microbiome Research. Frontiers in microbiology, 8102. https://doi.org/10.3389/fmicb.2017.00102
Menke, Sebastian, et al. "Home-Made Cost Effective Preservation Buffer Is a Better Alternative to Commercial Preservation Methods for Microbiome Research." Frontiers in microbiology vol. 8 (2017): 102. doi: https://doi.org/10.3389/fmicb.2017.00102
Menke S, Gillingham MA, Wilhelm K, Sommer S. Home-Made Cost Effective Preservation Buffer Is a Better Alternative to Commercial Preservation Methods for Microbiome Research. Front Microbiol. 2017 Jan 31;8:102. doi: 10.3389/fmicb.2017.00102. eCollection 2017. PMID: 28197142; PMCID: PMC5281576.
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Front Microbiol. 2017 Jan 31;8:102. doi: 10.3389/fmicb.2017.00102. eCollection 2017.

Home-Made Cost Effective Preservation Buffer Is a Better Alternative to Commercial Preservation Methods for Microbiome Research.

Frontiers in microbiology

Sebastian Menke, Mark A F Gillingham, Kerstin Wilhelm, Simone Sommer

Affiliations

  1. Institute of Evolutionary Ecology and Conservation Genomics, University of Ulm Ulm, Germany.

PMID: 28197142 PMCID: PMC5281576 DOI: 10.3389/fmicb.2017.00102

Abstract

The investigation of wildlife gastrointestinal microbiomes by next-generation sequencing approaches is a growing field in microbial ecology and conservation. Such studies often face difficulties in sample preservation if neither freezing facilities nor liquid nitrogen (LQN) are readily available. Thus, in order to prevent microbial community changes because of bacterial growth after sampling, preservation buffers need to be applied to samples. However, the amount of microbial community variation attributable to the different preservation treatments and potentially affecting biological interpretation is hardly known. Here, we sampled feces of 11 sheep (

Keywords: buffer; fecal samples; freezing; gut microbiome; next-generation sequencing; sample preservation; sheep; swabs

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