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Maltabe VA, Barka E, Kontonika M, et al. Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity. Stem Cells Int. 2016;2016:8305624doi: 10.1155/2016/8305624.
Maltabe, V. A., Barka, E., Kontonika, M., Florou, D., Kouvara-Pritsouli, M., Roumpi, M., Agathopoulos, S., Kolettis, T. M., & Kouklis, P. (2016). Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity. Stem cells international, 20168305624. https://doi.org/10.1155/2016/8305624
Maltabe, Violetta A, et al. "Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity." Stem cells international vol. 2016 (2016): 8305624. doi: https://doi.org/10.1155/2016/8305624
Maltabe VA, Barka E, Kontonika M, Florou D, Kouvara-Pritsouli M, Roumpi M, Agathopoulos S, Kolettis TM, Kouklis P. Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity. Stem Cells Int. 2016;2016:8305624. doi: 10.1155/2016/8305624. Epub 2016 Dec 22. PMID: 28101109; PMCID: PMC5215608.
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Stem Cells Int. 2016;2016:8305624. doi: 10.1155/2016/8305624. Epub 2016 Dec 22.

Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity.

Stem cells international

Violetta A Maltabe, Eleonora Barka, Marianthi Kontonika, Dimitra Florou, Maria Kouvara-Pritsouli, Maria Roumpi, Simeon Agathopoulos, Theofilos M Kolettis, Panos Kouklis

Affiliations

  1. Laboratory of Biology, Medical School, University of Ioannina, Ioannina, Greece; Department of Biomedical Research, Institute of Molecular Biology & Biotechnology, Foundation of Research and Technology-Hellas, University Campus, 45110 Ioannina, Greece.
  2. Ceramics and Composites Laboratory Department of Materials Science and Engineering, University of Ioannina, Ioannina, Greece.
  3. Department of Cardiology, University of Ioannina Medical School, Ioannina, Greece; Cardiovascular Research Institute, Ioannina, Greece.
  4. Laboratory of Biology, Medical School, University of Ioannina, Ioannina, Greece.

PMID: 28101109 PMCID: PMC5215608 DOI: 10.1155/2016/8305624

Abstract

Embryonic Stem (ES) or induced Pluripotent Stem (iPS) cells are important sources for cardiomyocyte generation, targeted for regenerative therapies. Several in vitro protocols are currently utilized for their differentiation, but the value of cell-based approaches remains unclear. Here, we characterized a cardiovascular progenitor population derived during ES differentiation, after selection based on VE-cadherin promoter (Pvec) activity. ESCs were genetically modified with an episomal vector, allowing the expression of puromycin resistance gene, under Pvec activity. Puromycin-surviving cells displayed cardiac and endothelial progenitor cells characteristics. Expansion and self-renewal of this cardiac and endothelial dual-progenitor population (CEDP) were achieved by Wnt/

Conflict of interest statement

The authors declare that there is no conflict of interests regarding the publication of this paper.

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