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Tafesse FG, Strijbis K, Ploegh HL. Quantitative Analysis of Cellular Diacylglycerol Content. Bio Protoc. 2014;4(15)doi: 10.21769/BioProtoc.1202.
Tafesse, F. G., Strijbis, K., & Ploegh, H. L. (2014). Quantitative Analysis of Cellular Diacylglycerol Content. Bio-protocol, 4(15), . https://doi.org/10.21769/BioProtoc.1202
Tafesse, Fikadu G, et al. "Quantitative Analysis of Cellular Diacylglycerol Content." Bio-protocol vol. 4,15 (2014). doi: https://doi.org/10.21769/BioProtoc.1202
Tafesse FG, Strijbis K, Ploegh HL. Quantitative Analysis of Cellular Diacylglycerol Content. Bio Protoc. 2014 Aug 05;4(15). doi: 10.21769/BioProtoc.1202. PMID: 28133622; PMCID: PMC5270654.
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Bio Protoc. 2014 Aug 05;4(15). doi: 10.21769/BioProtoc.1202.

Quantitative Analysis of Cellular Diacylglycerol Content.

Bio-protocol

Fikadu G Tafesse, Karin Strijbis, Hidde L Ploegh

Affiliations

  1. Whitehead Institute, Whitehead Institute of MIT, Cambridge, USA.

PMID: 28133622 PMCID: PMC5270654 DOI: 10.21769/BioProtoc.1202

Abstract

Diacylglycerol (DAG) is a bioactive lipid with diverse biological roles. DAG transiently accumulates in a membrane upon receipt of an appropriate stimulus that activates phospholipase C to cleave phospholipids. The resulting hydrolysis product DAG binds to proteins such as protein kinase C to initiate a variety of downstream cellular processes. DAG kinases attenuate such responses by converting DAG to phosphatidic acid. This protocol describes an assay designed to quantify cellular DAG levels. The assay exploits the enzymatic conversion of DAG (

References

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  3. J Biol Chem. 1986 Jul 5;261(19):8597-600 - PubMed

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