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Subramaniyan R, Marwaha N, Jain A, et al. Factors affecting the quality of cryoprecipitate. Asian J Transfus Sci. 2017;11(1):33-39doi: 10.4103/0973-6247.200778.
Subramaniyan, R., Marwaha, N., Jain, A., & Ahluwalia, J. (2017). Factors affecting the quality of cryoprecipitate. Asian journal of transfusion science, 11(1), 33-39. https://doi.org/10.4103/0973-6247.200778
Subramaniyan, Rajeswari, et al. "Factors affecting the quality of cryoprecipitate." Asian journal of transfusion science vol. 11,1 (2017): 33-39. doi: https://doi.org/10.4103/0973-6247.200778
Subramaniyan R, Marwaha N, Jain A, Ahluwalia J. Factors affecting the quality of cryoprecipitate. Asian J Transfus Sci. 2017 Jan-Jun;11(1):33-39. doi: 10.4103/0973-6247.200778. PMID: 28316438; PMCID: PMC5345278.
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Asian J Transfus Sci. 2017 Jan-Jun;11(1):33-39. doi: 10.4103/0973-6247.200778.

Factors affecting the quality of cryoprecipitate.

Asian journal of transfusion science

Rajeswari Subramaniyan, Neelam Marwaha, Ashish Jain, Jasmina Ahluwalia

Affiliations

  1. Department of Transfusion Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
  2. Department of Hematology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

PMID: 28316438 PMCID: PMC5345278 DOI: 10.4103/0973-6247.200778

Abstract

BACKGROUND: Many variables affect the quality of cryoprecipitate (CRYO). We investigated the effect of freezing techniques and ABO blood groups on the quality of CRYO with respect to factor VIII: C and fibrinogen levels.

MATERIALS AND METHODS: Ninety-six whole blood units each collected from in-house (Group I) and blood donation camps outside the hospital premises (Group II) were processed for CRYO preparation. Within each group, half the number of plasma units was frozen using blast freezer and another half using the conventional freezer. The CRYOs from blood groups A, B, and O were equally distributed, i.e. 32 within each of the Groups I and II. The fibrinogen and factor VIII: C levels in CRYO were analyzed using single-stage clotting assay.

RESULTS: In Group I, the mean ± standard deviation percentage recovery of factor VIII levels in CRYO prepared using the conventional freezer and blast freezer were 58.5% ±16.2% and 66.7% ±16.4%, respectively, and in Group II, it was 55.3% ±17.6% and 70.4% ±13.4%, respectively. Recovery of factor VIII was higher in CRYO prepared using blast freezer than that of CRYO prepared using conventional freezer (

CONCLUSION: The factor VIII recovery in CRYO improves significantly with higher baseline factor VIII: C levels, blood group A donor, and rapid freezing using blast freezer. Rapid freezing also increases the fibrinogen yield.

Keywords: ABO blood groups; blast freezer; cryoprecipitate; factor VIII: C; fibrinogen

Conflict of interest statement

There are no conflicts of interest.

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