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Muharram MM, Abdel-Kader MS. Utilization of gel electrophoreses for the quantitative estimation of digestive enzyme papain. Saudi Pharm J. 2016;25(3):359-364doi: 10.1016/j.jsps.2016.09.002.
Muharram, M. M., & Abdel-Kader, M. S. (2017). Utilization of gel electrophoreses for the quantitative estimation of digestive enzyme papain. Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society, 25(3), 359-364. https://doi.org/10.1016/j.jsps.2016.09.002
Muharram, Magdy M, and Abdel-Kader, Maged S. "Utilization of gel electrophoreses for the quantitative estimation of digestive enzyme papain." Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society vol. 25,3 (2017): 359-364. doi: https://doi.org/10.1016/j.jsps.2016.09.002
Muharram MM, Abdel-Kader MS. Utilization of gel electrophoreses for the quantitative estimation of digestive enzyme papain. Saudi Pharm J. 2017 Mar;25(3):359-364. doi: 10.1016/j.jsps.2016.09.002. Epub 2016 Sep 14. PMID: 28344489; PMCID: PMC5357110.
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Saudi Pharm J. 2017 Mar;25(3):359-364. doi: 10.1016/j.jsps.2016.09.002. Epub 2016 Sep 14.

Utilization of gel electrophoreses for the quantitative estimation of digestive enzyme papain.

Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society

Magdy M Muharram, Maged S Abdel-Kader

Affiliations

  1. Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, 11942 Alkharj, Saudi Arabia; Department of Microbiology, College of Science, Al-Azhar University, Nasr City, 11884 Cairo, Egypt.
  2. Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, 11942 Alkharj, Saudi Arabia; Department of Pharmacognosy, College of Pharmacy, Alexandria University, Alexandria 21215, Egypt.

PMID: 28344489 PMCID: PMC5357110 DOI: 10.1016/j.jsps.2016.09.002

Abstract

SDS-PAGE densitometric method for analysis of papain in pharmaceutical formulations was developed and validated for the first time. Standard and samples were mixed with SDS sample buffer and denatured at 95 °C for 5 min and the gel was run at 20 mA and 200 V for 30-40 min in SDS-PAGE buffer. Gels were stained in Coomassie blue solution and distained by 5% methanol and 10% acetic acid. Destained gels were imaged and analyzed using the ChemiDoc™ XRS+ System. Bands of papain appeared at

Keywords: ICH guidelines; Papain; Quantitative; SDS-PAGE

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