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Res Pharm Sci. 2017 Feb;12(1):38-45. doi: 10.4103/1735-5362.199045.

Radioprotective effects of lentil sprouts against X-ray radiation.

Research in pharmaceutical sciences

Abbas Haghparast, Kamran Mansouri, Samane Moradi, Fatemeh Dadashi, Saeed Eliasi, Mahdi Sobhani, Kambiz Varmira

Affiliations

  1. Department of Medical Physics, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, I.R. Iran.
  2. Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, I.R. Iran.
  3. Research Center of Oils and Fats, Food and Drug Administration, Kermanshah University of Medical Sciences, Kermanshah, I.R. Iran.
  4. Radiation Oncology Center, Imam Reza Hospital, Kermanshah, I.R. Iran.

PMID: 28255312 PMCID: PMC5333478 DOI: 10.4103/1735-5362.199045

Abstract

The present study investigated the radioprotective efficacy of lentil (Lens culinaris) sprouts against X-ray radiation-induced cellular damage. Lentil seeds were dark germinated at low temperature and the sprout extract was prepared in PBS. Free radical scavenging of extract was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and then the radioprotective potency of extract (0 to 1000 μg/mL) on the lymphocyte cells was determined by lactate dehydrogenases assay. Moreover, micronuclei assay was assessed using the cytokinesis-block technique. The irradiations were performed using 6 MV X-ray beam. The value of IC

Keywords: Germination; Legumes; Radioprotective agents; X-Radiation

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