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Cariboni A, Conti L, Andrè V, et al. Establishment of a radial glia-like mouse fetal hypothalamic neural stem cell line (AC1) able to differentiate into neuroendocrine cells. Neurogenesis (Austin). 2014;1(1):e29950doi: 10.4161/neur.29950.
Cariboni, A., Conti, L., Andrè, V., Aprile, D., Zasso, J., & Maggi, R. (2014). Establishment of a radial glia-like mouse fetal hypothalamic neural stem cell line (AC1) able to differentiate into neuroendocrine cells. Neurogenesis (Austin, Tex.), 1(1), e29950. https://doi.org/10.4161/neur.29950
Cariboni, Anna, et al. "Establishment of a radial glia-like mouse fetal hypothalamic neural stem cell line (AC1) able to differentiate into neuroendocrine cells." Neurogenesis (Austin, Tex.) vol. 1,1 (2014): e29950. doi: https://doi.org/10.4161/neur.29950
Cariboni A, Conti L, Andrè V, Aprile D, Zasso J, Maggi R. Establishment of a radial glia-like mouse fetal hypothalamic neural stem cell line (AC1) able to differentiate into neuroendocrine cells. Neurogenesis (Austin). 2014 Jul 28;1(1):e29950. doi: 10.4161/neur.29950. eCollection 2014. PMID: 28255570; PMCID: PMC5322806.
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Neurogenesis (Austin). 2014 Jul 28;1(1):e29950. doi: 10.4161/neur.29950. eCollection 2014.

Establishment of a radial glia-like mouse fetal hypothalamic neural stem cell line (AC1) able to differentiate into neuroendocrine cells.

Neurogenesis (Austin, Tex.)

Anna Cariboni, Luciano Conti, Valentina Andrè, Davide Aprile, Jacopo Zasso, Roberto Maggi

Affiliations

  1. Dipartimento di Scienze Farmacologiche e Biomolecolari; Sez. Biomedicina e Endocrinologia; ?Università degli Studi di Milano; Milan, Italy; Centro Interuniversitario per lo studio delle Malattie della Riproduzione (CIRMAR); Università degli Studi di Milano; Milan, Italy.
  2. Centro per la Biologia Integrata (CIBIO); Università degli Studi di Trento; Trento, Italy.
  3. Dipartimento di Scienze Farmacologiche e Biomolecolari; Sez. Biomedicina e Endocrinologia; ?Università degli Studi di Milano; Milan, Italy.

PMID: 28255570 PMCID: PMC5322806 DOI: 10.4161/neur.29950

Abstract

The present study describes the generation and the characterization of a stable cell line of neural stem cells derived from embryonic mouse hypothalamus. These cells (AC1) grow as an adherent culture in defined serum-free medium and express typical markers of neurogenic radial glia and of hypothalamic precursors. After prolonged expansion, AC1 cells may be efficiently induced to differentiate into neurons and astroglial cells in vitro and start to express some hormonal neuropeptides, like TRH, CRH, and POMC. Based on the capabilities of AC1 cells to be stably expanded and to develop neuroendocrine lineages in vitro, these cells might represent a novel tool to elucidate the mechanisms involved in the development of the hypothalamus and in the specific differentiation of neuroendocrine neurons.

Keywords: development; hypothalamus; neuroendocrinology; stem cells

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