Display options
Cite
Roy S, Dasgupta A. The Effects of Altered Membrane Cholesterol Levels on Sodium Pump Activity in Subclinical Hypothyroidism. Endocrinol Metab (Seoul). 2017;32(1):129-139doi: 10.3803/EnM.2017.32.1.129.
Roy, S., & Dasgupta, A. (2017). The Effects of Altered Membrane Cholesterol Levels on Sodium Pump Activity in Subclinical Hypothyroidism. Endocrinology and metabolism (Seoul, Korea), 32(1), 129-139. https://doi.org/10.3803/EnM.2017.32.1.129
Roy, Suparna, and Dasgupta, Anindya. "The Effects of Altered Membrane Cholesterol Levels on Sodium Pump Activity in Subclinical Hypothyroidism." Endocrinology and metabolism (Seoul, Korea) vol. 32,1 (2017): 129-139. doi: https://doi.org/10.3803/EnM.2017.32.1.129
Roy S, Dasgupta A. The Effects of Altered Membrane Cholesterol Levels on Sodium Pump Activity in Subclinical Hypothyroidism. Endocrinol Metab (Seoul). 2017 Mar;32(1):129-139. doi: 10.3803/EnM.2017.32.1.129. Epub 2017 Feb 28. PMID: 28256112; PMCID: PMC5368112.
Copy Download .nbib Format: NLM
Share it on

Endocrinol Metab (Seoul). 2017 Mar;32(1):129-139. doi: 10.3803/EnM.2017.32.1.129. Epub 2017 Feb 28.

The Effects of Altered Membrane Cholesterol Levels on Sodium Pump Activity in Subclinical Hypothyroidism.

Endocrinology and metabolism (Seoul, Korea)

Suparna Roy, Anindya Dasgupta

Affiliations

  1. Department of Biochemistry, Calcutta National Medical College, Kolkata, India.
  2. Department of Biochemistry, Calcutta National Medical College, Kolkata, India. [email protected].

PMID: 28256112 PMCID: PMC5368112 DOI: 10.3803/EnM.2017.32.1.129

Abstract

BACKGROUND: Metabolic dysfunctions characteristic of overt hypothyroidism (OH) start at the early stage of subclinical hypothyroidism (SCH). Na⁺/K⁺-ATPase (the sodium pump) is a transmembrane enzyme that plays a vital role in cellular activities in combination with membrane lipids. We evaluated the effects of early changes in thyroid hormone and membrane cholesterol on sodium pump activity in SCH and OH patients.

METHODS: In 32 SCH patients, 35 OH patients, and 34 euthyroid patients, sodium pump activity and cholesterol levels in red blood cell membranes were measured. Serum thyroxine (T₄) and thyroid stimulating hormone (TSH) levels were measured using enzyme-linked immunosorbent assays. Differences in their mean values were analysed using post hoc analysis of variance. We assessed the dependence of the sodium pump on other metabolites by multiple regression analysis.

RESULTS: Sodium pump activity and membrane cholesterol were lower in both hypothyroid groups than in control group, OH group exhibiting lower values than SCH group. In SCH group, sodium pump activity showed a significant direct dependence on membrane cholesterol with an inverse relationship with serum TSH levels. In OH group, sodium pump activity depended directly on membrane cholesterol and serum T₄ levels. No dependence on serum cholesterol was observed in either case.

CONCLUSION: Despite the presence of elevated serum cholesterol in hypothyroidism, membrane cholesterol contributed significantly to maintain sodium pump activity in the cells. A critical reduction in membrane cholesterol levels heralds compromised enzyme activity, even in the early stage of hypothyroidism, and this can be predicted by elevated TSH levels alone, without any evident clinical manifestations.

Copyright © 2017 Korean Endocrine Society

Keywords: Hypothyroidism; Membrane lipids; Sodium-potassium-exchanging ATPase

Conflict of interest statement

No potential conflict of interest relevant to this article was reported.

References

  1. Metabolism. 2007 Aug;56(8):1104-10 - PubMed
  2. Indian Heart J. 2000 May-Jun;52(3):315-8 - PubMed
  3. Eur J Endocrinol. 2012 Nov;167(5):609-18 - PubMed
  4. Clin Chim Acta. 1991 May 31;199(1):59-67 - PubMed
  5. J Biol Chem. 2007 Apr 6;282(14):10585-93 - PubMed
  6. J Gen Physiol. 1971 Jun;57(6):710-22 - PubMed
  7. Biochim Biophys Acta. 2015 Sep;1848(9):1729-43 - PubMed
  8. Am J Physiol Cell Physiol. 2003 Jun;284(6):C1550-60 - PubMed
  9. Stroke. 2013 May;44(5):1446-8 - PubMed
  10. Indian J Physiol Pharmacol. 2001 Jul;45(3):296-304 - PubMed
  11. Arch Biochem Biophys. 2008 Jul 15;475(2):121-7 - PubMed
  12. Biochim Biophys Acta. 1980 Aug 4;600(2):530-41 - PubMed
  13. Biochim Biophys Acta. 1988 Apr 7;939(2):189-96 - PubMed
  14. Lipids. 1987 Mar;22(3):148-51 - PubMed
  15. J Clin Endocrinol Metab. 2015 Apr;100(4):E607-10 - PubMed
  16. Clin Endocrinol (Oxf). 2007 Apr;66(4):548-56 - PubMed
  17. Clin Endocrinol (Oxf). 2004 Jun;60(6):705-10 - PubMed
  18. Mymensingh Med J. 2015 Apr;24(2):379-84 - PubMed
  19. Thyroid. 2014 Jun;24(6):931-8 - PubMed
  20. Biochem Biophys Res Commun. 2016 Jul 22;476(2):75-81 - PubMed
  21. Biochem Mol Biol Int. 1996 Dec;40(6):1087-94 - PubMed
  22. J Matern Fetal Neonatal Med. 2015 Aug;28(12):1438-44 - PubMed
  23. Endocr Rev. 2005 Aug;26(5):704-28 - PubMed
  24. Am J Nephrol. 2000 May-Jun;20(3):225-31 - PubMed
  25. Cholesterol. 2014;2014:821686 - PubMed
  26. Metabolism. 1983 Jan;32(1):25-30 - PubMed
  27. J Lipid Res. 1997 Aug;38(8):1503-21 - PubMed
  28. Hepatology. 2010 Oct;52(4):1401-9 - PubMed
  29. J Membr Biol. 2016 Jun;249(3):251-9 - PubMed
  30. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2591-5 - PubMed
  31. J Clin Endocrinol Metab. 2007 May;92 (5):1715-23 - PubMed
  32. Klin Wochenschr. 1984 Jan 2;62(1):11-8 - PubMed
  33. Thyroid. 2002 Apr;12(4):287-93 - PubMed
  34. Eur J Endocrinol. 2001 Dec;145(6):705-10 - PubMed
  35. Mol Biol Cell. 2006 Jan;17(1):317-26 - PubMed
  36. J Clin Endocrinol Metab. 2002 Apr;87(4):1533-8 - PubMed
  37. Am J Physiol Cell Physiol. 2006 Oct;291(4):C569-78 - PubMed
  38. Clin Lab. 2011;57(9-10):719-24 - PubMed
  39. Thyroid. 2000 Sep;10(9):753-9 - PubMed

Publication Types