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Chmyrov A, Leutenegger M, Grotjohann T, et al. Achromatic light patterning and improved image reconstruction for parallelized RESOLFT nanoscopy. Sci Rep. 2017;7:44619doi: 10.1038/srep44619.
Chmyrov, A., Leutenegger, M., Grotjohann, T., Schönle, A., Keller-Findeisen, J., Kastrup, L., Jakobs, S., Donnert, G., Sahl, S. J., & Hell, S. W. (2017). Achromatic light patterning and improved image reconstruction for parallelized RESOLFT nanoscopy. Scientific reports, 744619. https://doi.org/10.1038/srep44619
Chmyrov, Andriy, et al. "Achromatic light patterning and improved image reconstruction for parallelized RESOLFT nanoscopy." Scientific reports vol. 7 (2017): 44619. doi: https://doi.org/10.1038/srep44619
Chmyrov A, Leutenegger M, Grotjohann T, Schönle A, Keller-Findeisen J, Kastrup L, Jakobs S, Donnert G, Sahl SJ, Hell SW. Achromatic light patterning and improved image reconstruction for parallelized RESOLFT nanoscopy. Sci Rep. 2017 Mar 20;7:44619. doi: 10.1038/srep44619. PMID: 28317930; PMCID: PMC5357911.
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Sci Rep. 2017 Mar 20;7:44619. doi: 10.1038/srep44619.

Achromatic light patterning and improved image reconstruction for parallelized RESOLFT nanoscopy.

Scientific reports

Andriy Chmyrov, Marcel Leutenegger, Tim Grotjohann, Andreas Schönle, Jan Keller-Findeisen, Lars Kastrup, Stefan Jakobs, Gerald Donnert, Steffen J Sahl, Stefan W Hell

Affiliations

  1. Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Faßberg 11, 37077 Göttingen, Germany.
  2. Abberior Instruments GmbH, Hans-Adolf-Krebs-Weg 1, 37077 Göttingen, Germany.
  3. University of Göttingen, Medical Faculty, Department of Neurology, Robert-Koch-Str. 40, 37075 Göttingen, Germany.

PMID: 28317930 PMCID: PMC5357911 DOI: 10.1038/srep44619

Abstract

Fluorescence microscopy is rapidly turning into nanoscopy. Among the various nanoscopy methods, the STED/RESOLFT super-resolution family has recently been expanded to image even large fields of view within a few seconds. This advance relies on using light patterns featuring substantial arrays of intensity minima for discerning features by switching their fluorophores between 'on' and 'off' states of fluorescence. Here we show that splitting the light with a grating and recombining it in the focal plane of the objective lens renders arrays of minima with wavelength-independent periodicity. This colour-independent creation of periodic patterns facilitates coaligned on- and off-switching and readout with combinations chosen from a range of wavelengths. Applying up to three such periodic patterns on the switchable fluorescent proteins Dreiklang and rsCherryRev1.4, we demonstrate highly parallelized, multicolour RESOLFT nanoscopy in living cells for ~100 × 100 μm

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