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Ghanizadeh Tabriz A, Mills CG, Mullins JJ, et al. Rapid Fabrication of Cell-Laden Alginate Hydrogel 3D Structures by Micro Dip-Coating. Front Bioeng Biotechnol. 2017;5:13doi: 10.3389/fbioe.2017.00013.
Ghanizadeh Tabriz, A., Mills, C. G., Mullins, J. J., Davies, J. A., & Shu, W. (2017). Rapid Fabrication of Cell-Laden Alginate Hydrogel 3D Structures by Micro Dip-Coating. Frontiers in bioengineering and biotechnology, 513. https://doi.org/10.3389/fbioe.2017.00013
Ghanizadeh Tabriz, Atabak, et al. "Rapid Fabrication of Cell-Laden Alginate Hydrogel 3D Structures by Micro Dip-Coating." Frontiers in bioengineering and biotechnology vol. 5 (2017): 13. doi: https://doi.org/10.3389/fbioe.2017.00013
Ghanizadeh Tabriz A, Mills CG, Mullins JJ, Davies JA, Shu W. Rapid Fabrication of Cell-Laden Alginate Hydrogel 3D Structures by Micro Dip-Coating. Front Bioeng Biotechnol. 2017 Feb 24;5:13. doi: 10.3389/fbioe.2017.00013. eCollection 2017. PMID: 28286747; PMCID: PMC5323421.
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Front Bioeng Biotechnol. 2017 Feb 24;5:13. doi: 10.3389/fbioe.2017.00013. eCollection 2017.

Rapid Fabrication of Cell-Laden Alginate Hydrogel 3D Structures by Micro Dip-Coating.

Frontiers in bioengineering and biotechnology

Atabak Ghanizadeh Tabriz, Christopher G Mills, John J Mullins, Jamie A Davies, Wenmiao Shu

Affiliations

  1. School of Engineering and Physical Sciences, Heriot-Watt University , Edinburgh , UK.
  2. Centre for Integrative Physiology, University of Edinburgh, Edinburgh, UK; Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, UK.
  3. Centre for Cardiovascular Science, University of Edinburgh , Edinburgh , UK.
  4. Centre for Integrative Physiology, University of Edinburgh, Edinburgh, UK; Centre for Synthetic Biology, University of Edinburgh, Edinburgh, UK.
  5. School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh, UK; Department of Biomedical Engineering, University of Strathclyde, Glasgow, UK.

PMID: 28286747 PMCID: PMC5323421 DOI: 10.3389/fbioe.2017.00013

Abstract

Development of a simple, straightforward 3D fabrication method to culture cells in 3D, without relying on any complex fabrication methods, remains a challenge. In this paper, we describe a new technique that allows fabrication of scalable 3D cell-laden hydrogel structures easily, without complex machinery: the technique can be done using only apparatus already available in a typical cell biology laboratory. The fabrication method involves micro dip-coating of cell-laden hydrogels covering the surface of a metal bar, into the cross-linking reagents calcium chloride or barium chloride to form hollow tubular structures. This method can be used to form single layers with thickness ranging from 126 to 220 µm or multilayered tubular structures. This fabrication method uses alginate hydrogel as the primary biomaterial and a secondary biomaterial can be added depending on the desired application. We demonstrate the feasibility of this method, with survival rate over 75% immediately after fabrication and normal responsiveness of cells within these tubular structures using mouse dermal embryonic fibroblast cells and human embryonic kidney 293 cells containing a tetracycline-responsive, red fluorescent protein (tHEK cells).

Keywords: alginate; biofabrication; cell-laden; hydrogel; micro dip-coating; vascular structures

References

  1. Angew Chem Int Ed Engl. 2015 Mar 23;54(13):3957-61 - PubMed
  2. Nat Mater. 2013 Jun;12(6):584-90 - PubMed
  3. Biofabrication. 2015 Jun 12;7(3):035002 - PubMed
  4. Biofabrication. 2013 Mar;5(1):015012 - PubMed
  5. Drug Discov Today. 2002 Feb 1;7(3):179-86 - PubMed
  6. J Biomed Mater Res A. 2014 Oct;102(10):3379-92 - PubMed
  7. Oncol Rep. 2015 Apr;33(4):1837-43 - PubMed
  8. Nat Biotechnol. 2016 Mar;34(3):312-9 - PubMed
  9. J Mech Behav Biomed Mater. 2015 Feb;42:19-25 - PubMed
  10. Biofabrication. 2015 Nov 04;7(4):044101 - PubMed
  11. Biomaterials. 2011 Apr;32(12):3233-43 - PubMed
  12. Biofabrication. 2012 Dec;4(4):045005 - PubMed
  13. Circulation. 2006 Jul 4;114(1 Suppl):I87-93 - PubMed
  14. Biomacromolecules. 2006 May;7(5):1471-80 - PubMed
  15. Biomaterials. 2007 Aug;28(24):3508-16 - PubMed
  16. Biomaterials. 2007 Dec;28(35):5369-80 - PubMed
  17. Adv Mater. 2012 Feb 14;24(7):890-6 - PubMed
  18. Biofabrication. 2013 Mar;5(1):015013 - PubMed
  19. Sci Rep. 2016 Feb 09;6:20664 - PubMed
  20. Biofabrication. 2014 Sep;6(3):035001 - PubMed
  21. Semin Cancer Biol. 2005 Oct;15(5):405-12 - PubMed
  22. Biofabrication. 2015 Apr 14;7(2):025003 - PubMed
  23. Assay Drug Dev Technol. 2014 May;12(4):207-18 - PubMed
  24. Biotechnol Bioeng. 2006 Sep 5;95(1):1-7 - PubMed
  25. Biofabrication. 2016 Jan 08;8(1):013001 - PubMed
  26. Biofabrication. 2015 Feb 18;7(1):015010 - PubMed
  27. ACS Appl Mater Interfaces. 2010 Jul;2(7):1963-72 - PubMed
  28. FASEB J. 1998 Jan;12(1):47-56 - PubMed
  29. Biotechnol Bioeng. 2013 Feb;110(2):563-72 - PubMed
  30. Biofabrication. 2013 Sep;5(3):035001 - PubMed
  31. Biofabrication. 2015 Dec 21;7(4):045012 - PubMed
  32. J Funct Biomater. 2013 Jul 10;4(3):114-61 - PubMed
  33. Biofabrication. 2015 Jan 06;7(1):011001 - PubMed
  34. Biofabrication. 2014 Sep;6(3):035022 - PubMed
  35. Biofabrication. 2015 Oct 21;7(4):044102 - PubMed
  36. Biomaterials. 2011 Sep;32(26):6204-12 - PubMed

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