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Slotved HC, Hoffmann S. Evaluation of procedures for typing of group B . PeerJ. 2017;5:e3105doi: 10.7717/peerj.3105.
Slotved, H. C., & Hoffmann, S. (2017). Evaluation of procedures for typing of group B . PeerJ, 5e3105. https://doi.org/10.7717/peerj.3105
Slotved, Hans-Christian, and Hoffmann, Steen. "Evaluation of procedures for typing of group B ." PeerJ vol. 5 (2017): e3105. doi: https://doi.org/10.7717/peerj.3105
Slotved HC, Hoffmann S. Evaluation of procedures for typing of group B . PeerJ. 2017 Mar 16;5:e3105. doi: 10.7717/peerj.3105. eCollection 2017. PMID: 28321367; PMCID: PMC5357338.
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PeerJ. 2017 Mar 16;5:e3105. doi: 10.7717/peerj.3105. eCollection 2017.

Evaluation of procedures for typing of group B .

PeerJ

Hans-Christian Slotved, Steen Hoffmann

Affiliations

  1. Neisseria and Streptococcus Reference Laboratory, Department of Microbiology and Infection Control, Statens Serum Institut , Copenhagen , Denmark.

PMID: 28321367 PMCID: PMC5357338 DOI: 10.7717/peerj.3105

Abstract

BACKGROUND: This study evaluates two procedures for typing of

METHODS: A total of 616 clinical GBS isolates from 2010 to 2014 were tested with both a latex test and the LP test. Among these, 66 isolates were genotyped by PCR, including 41 isolates that were phenotypically NT.

RESULTS: The latex test provided a serotype for 83.8% of the isolates (95% CI [80.7-86.6]) compared to 87.5% (95% CI [84.6-90.0]) obtained by the LP method. The two assays provided identical capsular identification for all sero-typeable isolates (excluding NT isolates). The PCR assay provided a genotype designation to the 41 isolates defined as phenotypically NT isolates.

DISCUSSION: We found that the latex test showed a slightly lower identification percentage than the LP test. Our recommendation is to use the latex agglutination as the routine primary assay for GBS surveillance, and then use the more labour intensive precipitation test on the NT isolates to increase the serotyping rate. A genotype could be assigned to all the phenotypically NT isolates, however, as a consequence genotyping will overestimate the coverage from possible future capsular polysaccharide based GBS vaccines.

Keywords: Group B Streptococcus; Lancefield precipitation; Latex test; Serotyping

Conflict of interest statement

The authors declare there are no competing interests.

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