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3 Biotech. 2013 Feb;3(1):1-9. doi: 10.1007/s13205-012-0071-7. Epub 2012 Jun 06.

Enzyme immobilization: an overview on techniques and support materials.

3 Biotech

Sumitra Datta, L Rene Christena, Yamuna Rani Sriramulu Rajaram

Affiliations

  1. School of Chemical and Biotechnology, Shanmuga Arts, Science, Technology and Research Academy (SASTRA) University, Tirumalaisamudram, Thanjavur, 613401, Tamilnadu, India. [email protected].
  2. School of Chemical and Biotechnology, Shanmuga Arts, Science, Technology and Research Academy (SASTRA) University, Tirumalaisamudram, Thanjavur, 613401, Tamilnadu, India.

PMID: 28324347 PMCID: PMC3563746 DOI: 10.1007/s13205-012-0071-7

Abstract

The current demands of the world's biotechnological industries are enhancement in enzyme productivity and development of novel techniques for increasing their shelf life. These requirements are inevitable to facilitate large-scale and economic formulation. Enzyme immobilization provides an excellent base for increasing availability of enzyme to the substrate with greater turnover over a considerable period of time. Several natural and synthetic supports have been assessed for their efficiency for enzyme immobilization. Nowadays, immobilized enzymes are preferred over their free counterpart due to their prolonged availability that curtails redundant downstream and purification processes. Future investigations should endeavor at adopting logistic and sensible entrapment techniques along with innovatively modified supports to improve the state of enzyme immobilization and provide new perspectives to the industrial sector.

Keywords: Applications; Enzyme immobilization; Supports; Techniques

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