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Subathra Devi C, Saini A, Rastogi S, et al. Strain improvement and optimization studies for enhanced production of erythromycin in bagasse based medium using Saccharopolyspora erythraea MTCC 1103. 3 Biotech. 2013;5(1):23-31doi: 10.1007/s13205-013-0186-5.
Subathra Devi, C., Saini, A., Rastogi, S., Jemimah Naine, S., & Mohanasrinivasan, V. (2015). Strain improvement and optimization studies for enhanced production of erythromycin in bagasse based medium using Saccharopolyspora erythraea MTCC 1103. 3 Biotech, 5(1), 23-31. https://doi.org/10.1007/s13205-013-0186-5
Subathra Devi, C, et al. "Strain improvement and optimization studies for enhanced production of erythromycin in bagasse based medium using Saccharopolyspora erythraea MTCC 1103." 3 Biotech vol. 5,1 (2015): 23-31. doi: https://doi.org/10.1007/s13205-013-0186-5
Subathra Devi C, Saini A, Rastogi S, Jemimah Naine S, Mohanasrinivasan V. Strain improvement and optimization studies for enhanced production of erythromycin in bagasse based medium using Saccharopolyspora erythraea MTCC 1103. 3 Biotech. 2015 Feb;5(1):23-31. doi: 10.1007/s13205-013-0186-5. Epub 2013 Dec 18. PMID: 28324355; PMCID: PMC4327752.
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3 Biotech. 2015 Feb;5(1):23-31. doi: 10.1007/s13205-013-0186-5. Epub 2013 Dec 18.

Strain improvement and optimization studies for enhanced production of erythromycin in bagasse based medium using Saccharopolyspora erythraea MTCC 1103.

3 Biotech

C Subathra Devi, Anuj Saini, Shubham Rastogi, S Jemimah Naine, V Mohanasrinivasan

Affiliations

  1. Industrial Biotechnology Division, School of Biosciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India. [email protected].
  2. Industrial Biotechnology Division, School of Biosciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.

PMID: 28324355 PMCID: PMC4327752 DOI: 10.1007/s13205-013-0186-5

Abstract

In the present study, Saccharopolyspora erythraea MTCC 1103 was used for the enhanced production of erythromycin. To enhance the yield of erythromycin, effects of various parameters such as bagasse concentration, organic nitrogen source, inorganic nitrogen source, pH and temperature were analysed. It was found that bagasse can be used as an alternate carbon source in erythromycin production medium. Erythromycin production in the new formulation of bagasse based medium was found to be 512 mg/L which was 28 % higher than glucose based medium. Strain improvement was done by random UV-mutagenesis. When compared to wild type strain, mutant strain showed 40 % higher yield in production medium. Erythromycin potency assay and HPLC analysis were performed to confirm the presence of erythromycin in the partially purified samples. These optimized conditions could be used for the commercial production of this unique antibiotic which gave significant industrial perspectives.

Keywords: Bagasse; Erythromycin; Saccharopolyspora erythraea; UV-mutagenesis

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