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Basu M, Paichha M, Swain B, et al. Modulation of TLR2, TLR4, TLR5, NOD1 and NOD2 receptor gene expressions and their downstream signaling molecules following thermal stress in the Indian major carp catla (Catla catla). 3 Biotech. 2015;5(6):1021-1030doi: 10.1007/s13205-015-0306-5.
Basu, M., Paichha, M., Swain, B., Lenka, S. S., Singh, S., Chakrabarti, R., & Samanta, M. (2015). Modulation of TLR2, TLR4, TLR5, NOD1 and NOD2 receptor gene expressions and their downstream signaling molecules following thermal stress in the Indian major carp catla (Catla catla). 3 Biotech, 5(6), 1021-1030. https://doi.org/10.1007/s13205-015-0306-5
Basu, Madhubanti, et al. "Modulation of TLR2, TLR4, TLR5, NOD1 and NOD2 receptor gene expressions and their downstream signaling molecules following thermal stress in the Indian major carp catla (Catla catla)." 3 Biotech vol. 5,6 (2015): 1021-1030. doi: https://doi.org/10.1007/s13205-015-0306-5
Basu M, Paichha M, Swain B, Lenka SS, Singh S, Chakrabarti R, Samanta M. Modulation of TLR2, TLR4, TLR5, NOD1 and NOD2 receptor gene expressions and their downstream signaling molecules following thermal stress in the Indian major carp catla (Catla catla). 3 Biotech. 2015 Dec;5(6):1021-1030. doi: 10.1007/s13205-015-0306-5. Epub 2015 May 16. PMID: 28324409; PMCID: PMC4624144.
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3 Biotech. 2015 Dec;5(6):1021-1030. doi: 10.1007/s13205-015-0306-5. Epub 2015 May 16.

Modulation of TLR2, TLR4, TLR5, NOD1 and NOD2 receptor gene expressions and their downstream signaling molecules following thermal stress in the Indian major carp catla (Catla catla).

3 Biotech

Madhubanti Basu, Mahismita Paichha, Banikalyan Swain, Saswati S Lenka, Samarpal Singh, Rina Chakrabarti, Mrinal Samanta

Affiliations

  1. Fish Health Management Division, Central Institute of Freshwater Aquaculture (CIFA), Kausalyaganga, Bhubaneswar, 751002, Orissa, India.
  2. Aqua Research Lab, Department of Zoology, University of Delhi, Delhi, 110007, India.
  3. Fish Health Management Division, Central Institute of Freshwater Aquaculture (CIFA), Kausalyaganga, Bhubaneswar, 751002, Orissa, India. [email protected].

PMID: 28324409 PMCID: PMC4624144 DOI: 10.1007/s13205-015-0306-5

Abstract

Toll-like receptors (TLRs) and nucleotide binding and oligomerization domain (NOD) receptors are pattern recognition receptors (PRRs) that recognize pathogen-associated molecular patterns (PAMPs) and play crucial role in innate immunity. In addition to PAMPs, PRRs recognize endogenous molecules released from damaged tissue or dead cells [damage-associated molecular patterns (DAMPs)] and activate signaling cascades to induce inflammatory processes. In the aquatic environment, large variation in seasonal and diurnal water temperature causes heat and cold stresses in fish, resulting in tissue injury and mortality of fish. In the Indian subcontinent, catla (Catla catla) is an economically important freshwater fish species and is prone to thermal stresses. To investigate the response of pattern recognition receptors in thermal stress, we analyzed TLRs (TLR2, TLR4 and TLR5) and NOD (NOD1 and NOD2) receptors gene expression in catla following heat and cold stress. Analysis of tissue samples (gill, liver, kidney and blood) of the thermal stressed and control fish by quantitative real-time PCR (qRT-PCR) assay revealed significant (p < 0.05) induction of TLR2, TLR4 and NOD2 gene expression in majority of the tested tissues of the treated fish as compared to the control. The expression of TLR5 and NOD1 gene was also induced in the heat and cold stressed fish, but mostly restricted in the blood. The downstream signaling molecule of TLR and NOD signaling pathway viz., MyD88 (myeloid differentiation primary response gene 88) and RICK (receptor interacting serine-threonine protein kinase-2) was also induced in the thermal stressed fish suggesting the engagement of TLR and NOD signaling pathway during thermal stress.

Keywords: Catla catla; NOD; TLR; Thermal stress; Water temperature

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