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Pathak KV, Keharia H. Application of extracellular lipopeptide biosurfactant produced by endophytic Bacillus subtilis K1 isolated from aerial roots of banyan (Ficus benghalensis) in microbially enhanced oil recovery (MEOR). 3 Biotech. 2013;4(1):41-48doi: 10.1007/s13205-013-0119-3.
Pathak, K. V., & Keharia, H. (2014). Application of extracellular lipopeptide biosurfactant produced by endophytic Bacillus subtilis K1 isolated from aerial roots of banyan (Ficus benghalensis) in microbially enhanced oil recovery (MEOR). 3 Biotech, 4(1), 41-48. https://doi.org/10.1007/s13205-013-0119-3
Pathak, Khyati V, and Keharia, Hareshkumar. "Application of extracellular lipopeptide biosurfactant produced by endophytic Bacillus subtilis K1 isolated from aerial roots of banyan (Ficus benghalensis) in microbially enhanced oil recovery (MEOR)." 3 Biotech vol. 4,1 (2014): 41-48. doi: https://doi.org/10.1007/s13205-013-0119-3
Pathak KV, Keharia H. Application of extracellular lipopeptide biosurfactant produced by endophytic Bacillus subtilis K1 isolated from aerial roots of banyan (Ficus benghalensis) in microbially enhanced oil recovery (MEOR). 3 Biotech. 2014 Feb;4(1):41-48. doi: 10.1007/s13205-013-0119-3. Epub 2013 Feb 12. PMID: 28324457; PMCID: PMC3909566.
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3 Biotech. 2014 Feb;4(1):41-48. doi: 10.1007/s13205-013-0119-3. Epub 2013 Feb 12.

Application of extracellular lipopeptide biosurfactant produced by endophytic Bacillus subtilis K1 isolated from aerial roots of banyan (Ficus benghalensis) in microbially enhanced oil recovery (MEOR).

3 Biotech

Khyati V Pathak, Hareshkumar Keharia

Affiliations

  1. BRD School of Biosciences, Sardar Patel Maidan, Satellite Campus, Sardar Patel University, Vadtal Road, P.O. Box 39, Vallabh Vidyangar, 388120, Gujarat, India.
  2. BRD School of Biosciences, Sardar Patel Maidan, Satellite Campus, Sardar Patel University, Vadtal Road, P.O. Box 39, Vallabh Vidyangar, 388120, Gujarat, India. [email protected].

PMID: 28324457 PMCID: PMC3909566 DOI: 10.1007/s13205-013-0119-3

Abstract

Bacillus subtilis K1 isolated from aerial roots of banyan tree secreted mixture of surfactins, iturins and fengycins with high degree of heterogeneity. The extracellular extract consisting of mixture of these cyclic lipopeptides exhibited very good emulsification activity as well as excellent emulsion stability. The culture accumulated maximum surfactant up to 48 h of growth during batch fermentation in Luria broth. The emulsion of hexane, heptane and octane prepared using 48-h-old culture supernatant of B. subtilis K1 remained stable up to 2 days while emulsion of four stroke engine oil remained stable for more than a year. The critical micelle concentration of crude lipopeptide biosurfactant extracted by acid precipitation from 48-h-old fermentation broth of B. subtilis K1 was found to be 20.5 μg/mL. The biosurfactant activity was found to be stable at 100 °C for 2 h, over a pH range of 6-12 h and over an NaCl concentration up to 10 % (w/v). The application of biosurfactant on laboratory scale sand pack column saturated with four stroke engine oil resulted in ~43 % enhanced oil recovery, suggesting its suitability in microbially enhanced oil recovery.

Keywords: Bacillus subtilis; Critical micelle concentration; Emulsifying activity; Lipopeptide biosurfactants; MEOR

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