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Bhatia SK, Mehta PK, Bhatia RK, et al. Simultaneous purification of nitrile hydratase and amidase of Alcaligenes sp. MTCC 10674. 3 Biotech. 2013;4(4):375-381doi: 10.1007/s13205-013-0163-z.
Bhatia, S. K., Mehta, P. K., Bhatia, R. K., & Bhalla, T. C. (2014). Simultaneous purification of nitrile hydratase and amidase of Alcaligenes sp. MTCC 10674. 3 Biotech, 4(4), 375-381. https://doi.org/10.1007/s13205-013-0163-z
Bhatia, S K, et al. "Simultaneous purification of nitrile hydratase and amidase of Alcaligenes sp. MTCC 10674." 3 Biotech vol. 4,4 (2014): 375-381. doi: https://doi.org/10.1007/s13205-013-0163-z
Bhatia SK, Mehta PK, Bhatia RK, Bhalla TC. Simultaneous purification of nitrile hydratase and amidase of Alcaligenes sp. MTCC 10674. 3 Biotech. 2014 Aug;4(4):375-381. doi: 10.1007/s13205-013-0163-z. Epub 2013 Aug 27. PMID: 28324474; PMCID: PMC4145624.
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3 Biotech. 2014 Aug;4(4):375-381. doi: 10.1007/s13205-013-0163-z. Epub 2013 Aug 27.

Simultaneous purification of nitrile hydratase and amidase of Alcaligenes sp. MTCC 10674.

3 Biotech

S K Bhatia, P K Mehta, R K Bhatia, T C Bhalla

Affiliations

  1. Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla, 171005, India.
  2. Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla, 171005, India. [email protected].

PMID: 28324474 PMCID: PMC4145624 DOI: 10.1007/s13205-013-0163-z

Abstract

Alcaligenes sp. MTCC 10674 has a bienzymatic system for the hydrolysis of nitriles. The nitrile hydratase and amidase have been purified simultaneously to homogeneity using a combination of (NH)

Keywords: Alcaligenes sp. MTCC 10674; Amidase; Bienzymatic; Nitrile hydratase

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