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Bhatia SK, Kumar N, Bhatia RK. Stepwise bioprocess for exopolysaccharide production using potato starch as carbon source. 3 Biotech. 2014;5(5):735-739doi: 10.1007/s13205-014-0273-2.
Bhatia, S. K., Kumar, N., & Bhatia, R. K. (2015). Stepwise bioprocess for exopolysaccharide production using potato starch as carbon source. 3 Biotech, 5(5), 735-739. https://doi.org/10.1007/s13205-014-0273-2
Bhatia, Shashi Kant, et al. "Stepwise bioprocess for exopolysaccharide production using potato starch as carbon source." 3 Biotech vol. 5,5 (2015): 735-739. doi: https://doi.org/10.1007/s13205-014-0273-2
Bhatia SK, Kumar N, Bhatia RK. Stepwise bioprocess for exopolysaccharide production using potato starch as carbon source. 3 Biotech. 2015 Oct;5(5):735-739. doi: 10.1007/s13205-014-0273-2. Epub 2014 Dec 23. PMID: 28324523; PMCID: PMC4569638.
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3 Biotech. 2015 Oct;5(5):735-739. doi: 10.1007/s13205-014-0273-2. Epub 2014 Dec 23.

Stepwise bioprocess for exopolysaccharide production using potato starch as carbon source.

3 Biotech

Shashi Kant Bhatia, Narinder Kumar, Ravi Kant Bhatia

Affiliations

  1. Department of Biotechnology, Indian Institute of Education, Ghanahatti, Shimla, 171011, India. [email protected].
  2. Department of Biotechnology, Himachal Pradesh University, Shimla, 171005, India. [email protected].
  3. Department of Biotechnology, Indian Institute of Education, Ghanahatti, Shimla, 171011, India.
  4. Department of Biotechnology, Himachal Pradesh University, Shimla, 171005, India.

PMID: 28324523 PMCID: PMC4569638 DOI: 10.1007/s13205-014-0273-2

Abstract

Xanthan gum is a biopolymer produced by Xanthomonas sp. XC6. In this study, xanthan gum is produced from potato starch using a stepwise bioprocess design. Potato starch is hydrolyzed using Bacillus sp. having amylase activity and 30.2 g/L reducing sugar was released, while Xanthomonas sp. XC6 can release only 14.5 g/L. Bacillus sp. hydrolyzed potato starch extract was further used as a carbon source for xanthan gum biosynthesis using Xanthomonas sp. XC6. Yeast extract acts as the best nitrogen source, and 10.0 g/L xanthan gum was recovered. Downstreaming process after stepwise bioprocess resulted in 17.4 g/L xanthan gum production, which is 2.8 times higher as compared to single step process.

Keywords: Biopolymer; Biosynthesis; Downstreaming process; Xanthan gum

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