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Shahbaaz M, Ahmad F, Hassan MI. Structure-based function analysis of putative conserved proteins with isomerase activity from Haemophilus influenzae. 3 Biotech. 2014;5(5):741-763doi: 10.1007/s13205-014-0274-1.
Shahbaaz, M., Ahmad, F., & Hassan, M. I. (2015). Structure-based function analysis of putative conserved proteins with isomerase activity from Haemophilus influenzae. 3 Biotech, 5(5), 741-763. https://doi.org/10.1007/s13205-014-0274-1
Shahbaaz, Mohd, et al. "Structure-based function analysis of putative conserved proteins with isomerase activity from Haemophilus influenzae." 3 Biotech vol. 5,5 (2015): 741-763. doi: https://doi.org/10.1007/s13205-014-0274-1
Shahbaaz M, Ahmad F, Hassan MI. Structure-based function analysis of putative conserved proteins with isomerase activity from Haemophilus influenzae. 3 Biotech. 2015 Oct;5(5):741-763. doi: 10.1007/s13205-014-0274-1. Epub 2014 Dec 28. PMID: 28324524; PMCID: PMC4569619.
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3 Biotech. 2015 Oct;5(5):741-763. doi: 10.1007/s13205-014-0274-1. Epub 2014 Dec 28.

Structure-based function analysis of putative conserved proteins with isomerase activity from Haemophilus influenzae.

3 Biotech

Mohd Shahbaaz, Faizan Ahmad, Md Imtaiyaz Hassan

Affiliations

  1. Department of Computer Science, Jamia Millia Islamia, Jamia Nagar, New Delhi, 110025, India.
  2. Center for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi, 110025, India.
  3. Center for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi, 110025, India. [email protected].

PMID: 28324524 PMCID: PMC4569619 DOI: 10.1007/s13205-014-0274-1

Abstract

Haemophilus influenzae, a Gram-negative bacterium and a member of the family Pasteurellaceae, causes chronic bronchitis, bacteremia, meningitis, etc. The H. influenzae is the first organism whose genome was completely sequenced and annotated. Here, we have extensively analyzed the genome of H. influenzae using available proteins structure and function analysis tools. The objective of this analysis is to assign a precise function to hypothetical proteins (HPs) whose functions are not determined so far. Function prediction of these proteins is helpful in precise understanding of mechanisms of pathogenesis and biochemical pathways important for selecting novel therapeutic target. After an extensive analysis of H. Influenzae genome we have found 13 HPs showing high level of sequence and structural similarity to the enzyme isomerase. Consequently, the structures of HPs have been modeled and analyzed to determine their precise functions. We found these HPs are alanine racemase, lysine 2, 3-aminomutase, topoisomerase DNA-binding C4 zinc finger, pseudouridine synthase B, C and E (Rlu B, C and E), hydroxypyruvate isomerase, nucleoside-diphosphate-sugar epimerase, amidophosphoribosyltransferase, aldose-1-epimerase, tautomerase/MIF, Xylose isomerase-like, have TIM barrel domain and sedoheptulose-7-phosphate isomerase like activity, signifying their corresponding functions in the H. influenzae. This work provides a better understanding of the role HPs with isomerase activities in the survival and pathogenesis of H. influenzae.

Keywords: Haemophilus influenza; Hypothetical protein; Isomerase activity; Structure analysis; Structure prediction; Structure–function relationship

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