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Thomas P, Sekhar AC, Upreti R, et al. Optimization of single plate-serial dilution spotting (SP-SDS) with sample anchoring as an assured method for bacterial and yeast cfu enumeration and single colony isolation from diverse samples. Biotechnol Rep (Amst). 2015;8:45-55doi: 10.1016/j.btre.2015.08.003.
Thomas, P., Sekhar, A. C., Upreti, R., Mujawar, M. M., & Pasha, S. S. (2015). Optimization of single plate-serial dilution spotting (SP-SDS) with sample anchoring as an assured method for bacterial and yeast cfu enumeration and single colony isolation from diverse samples. Biotechnology reports (Amsterdam, Netherlands), 845-55. https://doi.org/10.1016/j.btre.2015.08.003
Thomas, Pious, et al. "Optimization of single plate-serial dilution spotting (SP-SDS) with sample anchoring as an assured method for bacterial and yeast cfu enumeration and single colony isolation from diverse samples." Biotechnology reports (Amsterdam, Netherlands) vol. 8 (2015): 45-55. doi: https://doi.org/10.1016/j.btre.2015.08.003
Thomas P, Sekhar AC, Upreti R, Mujawar MM, Pasha SS. Optimization of single plate-serial dilution spotting (SP-SDS) with sample anchoring as an assured method for bacterial and yeast cfu enumeration and single colony isolation from diverse samples. Biotechnol Rep (Amst). 2015 Aug 20;8:45-55. doi: 10.1016/j.btre.2015.08.003. eCollection 2015 Dec. PMID: 28352572; PMCID: PMC4980700.
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Biotechnol Rep (Amst). 2015 Aug 20;8:45-55. doi: 10.1016/j.btre.2015.08.003. eCollection 2015 Dec.

Optimization of single plate-serial dilution spotting (SP-SDS) with sample anchoring as an assured method for bacterial and yeast cfu enumeration and single colony isolation from diverse samples.

Biotechnology reports (Amsterdam, Netherlands)

Pious Thomas, Aparna C Sekhar, Reshmi Upreti, Mohammad M Mujawar, Sadiq S Pasha

Affiliations

  1. Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake, Bangalore 560089, India.

PMID: 28352572 PMCID: PMC4980700 DOI: 10.1016/j.btre.2015.08.003

Abstract

We propose a simple technique for bacterial and yeast cfu estimations from diverse samples with no prior idea of viable counts, designated as single plate-serial dilution spotting (SP-SDS) with the prime recommendation of sample anchoring (10

Keywords: Agricultural biotechnology; CNA, cetrimide- nalixic acid- agar; Environmental biotechnology; Food microbiology; NA, nutrient agar; NB, nutrient broth; OD, optical density; PDA, potato dextrose agar; PP, polypropylene bag; PS, peptone-salt; Pour-plating; SATS, spotting- and- tilt- spreading; SP-SDS, single plate-serial dilution spotting; Spread-plating; cfu Estimation; cfu, colony forming units; tmtc, too many to count

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