Display options
Cite
Zhang J, Liu X, Ying YL, et al. High-bandwidth nanopore data analysis by using a modified hidden Markov model. Nanoscale. 2017;9(10):3458-3465doi: 10.1039/c6nr09135k.
Zhang, J., Liu, X., Ying, Y. L., Gu, Z., Meng, F. N., & Long, Y. T. (2017). High-bandwidth nanopore data analysis by using a modified hidden Markov model. Nanoscale, 9(10), 3458-3465. https://doi.org/10.1039/c6nr09135k
Zhang, Jianhua, et al. "High-bandwidth nanopore data analysis by using a modified hidden Markov model." Nanoscale vol. 9,10 (2017): 3458-3465. doi: https://doi.org/10.1039/c6nr09135k
Zhang J, Liu X, Ying YL, Gu Z, Meng FN, Long YT. High-bandwidth nanopore data analysis by using a modified hidden Markov model. Nanoscale. 2017 Mar 09;9(10):3458-3465. doi: 10.1039/c6nr09135k. PMID: 28232981.
Copy Download .nbib Format: NLM
Share it on

Nanoscale. 2017 Mar 09;9(10):3458-3465. doi: 10.1039/c6nr09135k.

High-bandwidth nanopore data analysis by using a modified hidden Markov model.

Nanoscale

Jianhua Zhang, Xiuling Liu, Yi-Lun Ying, Zhen Gu, Fu-Na Meng, Yi-Tao Long

Affiliations

  1. School of Information Science and Engineering, East China University of Science and Technology, Shanghai 200237, P.R. China. [email protected].
  2. Key Laboratory for Advanced Materials and Department of Chemistry, East China University of Science and Technology, Shanghai 200237, P.R. China. [email protected].

PMID: 28232981 DOI: 10.1039/c6nr09135k

Abstract

Nanopore-based sensing is an emerging analytical technique with a number of important applications, including single-molecule detection and DNA sequencing. In this paper, we developed a Modified Hidden Markov Model (MHMM) to analyze directly the raw (unfiltered) nanopore current blockade data, which significantly reduced the filtering-induced distortion of the nanopore events. Traditionally, prior to further analysis, the measured nanopore data need to be pre-filtered to supress the strong noises. Nonetheless, this would result in the distortion of the shape of the blockade current especially for rapid translocations and bumping blockades. The HMM has been proved to be robust with respect to highly noisy data and thus ideally suitable for processing raw nanopore data directly. Unfortunately, its performance is somehow sensitive to the initial parameters usually preset arbitrarily. To overcome this problem, we use the Fuzzy c-Means (FCM) algorithm to initialize the HMM parameters automatically. Then we use the Viterbi training algorithm to optimize the HMM. Finally, the application results on both the simulated and experimental data are presented to demonstrate the practicability of the developed method for accurate detection of the nanopore current blockade events. The proposed method enables detection of the nanopore events at the highest bandwidth of the commercial instruments to extract the true useful information about the single molecules under analysis.

Publication Types