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Schelkle KM, Schmid C, Yserentant K, et al. Cell Fixation by Light-Triggered Release of Glutaraldehyde. Angew Chem Int Ed Engl. 2017;56(17):4724-4728doi: 10.1002/anie.201612112.
Schelkle, K. M., Schmid, C., Yserentant, K., Bender, M., Wacker, I., Petzoldt, M., Hamburger, M., Herten, D. P., Wombacher, R., Schröder, R. R., & Bunz, U. H. (2017). Cell Fixation by Light-Triggered Release of Glutaraldehyde. Angewandte Chemie (International ed. in English), 56(17), 4724-4728. https://doi.org/10.1002/anie.201612112
Schelkle, Korwin M, et al. "Cell Fixation by Light-Triggered Release of Glutaraldehyde." Angewandte Chemie (International ed. in English) vol. 56,17 (2017): 4724-4728. doi: https://doi.org/10.1002/anie.201612112
Schelkle KM, Schmid C, Yserentant K, Bender M, Wacker I, Petzoldt M, Hamburger M, Herten DP, Wombacher R, Schröder RR, Bunz UH. Cell Fixation by Light-Triggered Release of Glutaraldehyde. Angew Chem Int Ed Engl. 2017 Apr 18;56(17):4724-4728. doi: 10.1002/anie.201612112. Epub 2017 Mar 22. PMID: 28328078.
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Angew Chem Int Ed Engl. 2017 Apr 18;56(17):4724-4728. doi: 10.1002/anie.201612112. Epub 2017 Mar 22.

Cell Fixation by Light-Triggered Release of Glutaraldehyde.

Angewandte Chemie (International ed. in English)

Korwin M Schelkle, Christopher Schmid, Klaus Yserentant, Markus Bender, Irene Wacker, Martin Petzoldt, Manuel Hamburger, Dirk-Peter Herten, Richard Wombacher, Rasmus R Schröder, Uwe H F Bunz

Affiliations

  1. Organisch-Chemisches Institut, Ruprecht-Karls-Universität Heidelberg, Im Neuenheimer Feld 270, 69120, Heidelberg, Germany.
  2. InnovationLab GmbH, Speyerer Strasse 4, 69115, Heidelberg, Germany.
  3. Center of Advanced Materials, Ruprecht-Karls-Universität Heidelberg, Im Neuenheimer Feld 225, 69120, Heidelberg, Germany.
  4. Physikalisch-Chemisches Institut, Ruprecht-Karls-Universität Heidelberg, Im Neuenheimer Feld 229, 69120, Heidelberg, Germany.
  5. CellNetworks, Single Molecule Spectroscopy, Ruprecht-Karls-Universität Heidelberg, Im Neuenheimer Feld 267, 69120, Heidelberg, Germany.
  6. Institut für Pharmazie und Molekulare Biotechnologie, Ruprecht-Karls-Universität Heidelberg, Im Neuenheimer Feld 364, 69120, Heidelberg, Germany.
  7. CellNetworks, Cryo Electron Microscopy, Universitätsklinikum Heidelberg, Im Neuenheimer Feld 267, 69120, Heidelberg, Germany.

PMID: 28328078 DOI: 10.1002/anie.201612112

Abstract

Chemical fixation of living cells for microscopy is commonly achieved by crosslinking of intracellular proteins with dialdehydes prior to examination. We herein report a photocleavable protecting group for glutaraldehyde that results in a light-triggered and membrane-permeable fixative, which is nontoxic prior to photocleavage. Lipophilic ester groups allow for diffusion across the cell membrane and intracellular accumulation after enzymatic hydrolysis. Irradiation with UV light releases glutaraldehyde. The in situ generated fixative crosslinks intracellular proteins and preserves and stabilizes the cell so that it is ready for microscopy. In contrast to conventional glutaraldehyde fixation, tissue autofluorescence does not increase after fixation. Caged glutaraldehyde may in future enable functional experiments on living cells under a light microscope in which events of interest can be stopped in spatially confined volumes at defined time points. Samples with individually stopped events could then later be analyzed in ultrastructural studies.

© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords: cell fixation; glutaraldehyde; microscopy; mitochondrial motion; photocleavage

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