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Front Bioeng Biotechnol. 2017 Feb 28;5:14. doi: 10.3389/fbioe.2017.00014. eCollection 2017.

PCR Primer Design for 16S rRNAs for Experimental Horizontal Gene Transfer Test in .

Frontiers in bioengineering and biotechnology

Kentaro Miyazaki, Mitsuharu Sato, Miyuki Tsukuda

Affiliations

  1. Department of Life Science and Biotechnology, Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan; Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, Japan.

PMID: 28293553 PMCID: PMC5329695 DOI: 10.3389/fbioe.2017.00014

Abstract

We recently demonstrated that the

Keywords: 16S rRNA; bacterial phylogeny; central pseudoknot; functional complementation; horizontal gene transfer; metagenome; molecular clock; ribosome

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