Display options
Cite
Maryam, Jaskani MJ, Awan FS, et al. Development of molecular method for sex identification in date palm (Phoenix dactylifera L.) plantlets using novel sex-linked microsatellite markers. 3 Biotech. 2016;6(1):22doi: 10.1007/s13205-015-0321-6.
Maryam, Jaskani, M. J., Awan, F. S., Ahmad, S., & Khan, I. A. (2016). Development of molecular method for sex identification in date palm (Phoenix dactylifera L.) plantlets using novel sex-linked microsatellite markers. 3 Biotech, 6(1), 22. https://doi.org/10.1007/s13205-015-0321-6
Maryam, et al. "Development of molecular method for sex identification in date palm (Phoenix dactylifera L.) plantlets using novel sex-linked microsatellite markers." 3 Biotech vol. 6,1 (2016): 22. doi: https://doi.org/10.1007/s13205-015-0321-6
Maryam, Jaskani MJ, Awan FS, Ahmad S, Khan IA. Development of molecular method for sex identification in date palm (Phoenix dactylifera L.) plantlets using novel sex-linked microsatellite markers. 3 Biotech. 2016 Jun;6(1):22. doi: 10.1007/s13205-015-0321-6. Epub 2016 Jan 09. PMID: 28330092; PMCID: PMC4706835.
Copy Download .nbib Format: NLM
Share it on

3 Biotech. 2016 Jun;6(1):22. doi: 10.1007/s13205-015-0321-6. Epub 2016 Jan 09.

Development of molecular method for sex identification in date palm (Phoenix dactylifera L.) plantlets using novel sex-linked microsatellite markers.

3 Biotech

Maryam, Muhammad Jafar Jaskani, Faisal Saeed Awan, Saeed Ahmad, Iqrar A Khan

Affiliations

  1. Institute of Horticultural Sciences, University of Agriculture Faisalabad, Faisalabad, 38000, Pakistan.
  2. Institute of Horticultural Sciences, University of Agriculture Faisalabad, Faisalabad, 38000, Pakistan. [email protected].
  3. Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, 38000, Pakistan.

PMID: 28330092 PMCID: PMC4706835 DOI: 10.1007/s13205-015-0321-6

Abstract

Microsatellite markers containing simple sequence repeats (SSRs) are a valuable tool for genetic analysis. Date palm is a dioecious and slow flowering and is very difficult to identify the gender of the trees until it reaches the reproductive age (5-10 years). A total of 12 microsatellite primers were used with 30 date palm samples, 14 parents (8 male + 6 females) and 16 progeny (developed from parents breeding) which showed that microsatellites were highly polymorphic, having a great number of alleles. A total of 124 alleles were characterized in 12 SSR loci. On average, there are 9.08 alleles per locus, with a range from 5 to 16 alleles, for primers mpdCIR15 and mpdCIR57, respectively. These primers produced 15 polymorphic loci specifically in male date palm samples and the seedlings harboring the unique fragments were further characterized as male plants. Increasingly, 38.46 % of these loci were scored as homozygous alleles while 61.53 % heterozygous allelic loci were determined. Primer mpdCIR48 produced a specific locus (250/250) in all male samples whereas the same locus was absent in female samples. Similarly, a locus of 300/310 bp reoccurred in 5 date palm male samples using marker DP-168 which indicated that these are the promising candidate marker to detect the sex in date palm seedlings at early stage. The data resulted from combination of 12 primers enabled the 16 seedling samples progeny (developed from parents breeding) of date palm cultivars to divide into two groups i.e., male and female regarding their sex expression comparative to the parents (male + female) using the principle coordinate analysis.

Keywords: Phoenix dactylifera L.; Polymorphism; SSR marker; Sex identification

References

  1. Curr Top Dev Biol. 1998;38:167-223 - PubMed
  2. Plant Mol Biol. 1992 Jan;18(2):315-25 - PubMed
  3. Ann Bot. 2010 Aug;106(2):255-66 - PubMed
  4. Hereditas. 2004;141(3):278-87 - PubMed
  5. Nat Biotechnol. 2011 May 29;29(6):521-7 - PubMed
  6. Nucleic Acids Res. 1990 Dec 25;18(24):7213-8 - PubMed
  7. Curr Biol. 1996 Feb 1;6(2):149-62 - PubMed
  8. Theor Appl Genet. 1994 Sep;89(1):9-13 - PubMed
  9. Theor Appl Genet. 1991 Dec;83(2):194-200 - PubMed
  10. BMC Plant Biol. 2012 Dec 15;12:237 - PubMed
  11. Genetica. 2008 Oct;134(2):251-60 - PubMed

Publication Types