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Neifar M, Chouchane H, Mahjoubi M, et al. Pseudomonas extremorientalis BU118: a new salt-tolerant laccase-secreting bacterium with biotechnological potential in textile azo dye decolourization. 3 Biotech. 2016;6(1):107doi: 10.1007/s13205-016-0425-7.
Neifar, M., Chouchane, H., Mahjoubi, M., Jaouani, A., & Cherif, A. (2016). Pseudomonas extremorientalis BU118: a new salt-tolerant laccase-secreting bacterium with biotechnological potential in textile azo dye decolourization. 3 Biotech, 6(1), 107. https://doi.org/10.1007/s13205-016-0425-7
Neifar, Mohamed, et al. "Pseudomonas extremorientalis BU118: a new salt-tolerant laccase-secreting bacterium with biotechnological potential in textile azo dye decolourization." 3 Biotech vol. 6,1 (2016): 107. doi: https://doi.org/10.1007/s13205-016-0425-7
Neifar M, Chouchane H, Mahjoubi M, Jaouani A, Cherif A. Pseudomonas extremorientalis BU118: a new salt-tolerant laccase-secreting bacterium with biotechnological potential in textile azo dye decolourization. 3 Biotech. 2016 Jun;6(1):107. doi: 10.1007/s13205-016-0425-7. Epub 2016 Apr 18. PMID: 28330177; PMCID: PMC4835423.
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3 Biotech. 2016 Jun;6(1):107. doi: 10.1007/s13205-016-0425-7. Epub 2016 Apr 18.

Pseudomonas extremorientalis BU118: a new salt-tolerant laccase-secreting bacterium with biotechnological potential in textile azo dye decolourization.

3 Biotech

Mohamed Neifar, Habib Chouchane, Mouna Mahjoubi, Atef Jaouani, Ameur Cherif

Affiliations

  1. BVBGR-LR11ES31, ISBST, University of Manouba, Biotechpole Sidi Thabet, 2020, Ariana, Tunisia. [email protected].
  2. BVBGR-LR11ES31, ISBST, University of Manouba, Biotechpole Sidi Thabet, 2020, Ariana, Tunisia.
  3. MBA-LR03ES03, FST, University of Tunis El Manar, Campus Universitaire, 2092, Tunis, Tunisia.

PMID: 28330177 PMCID: PMC4835423 DOI: 10.1007/s13205-016-0425-7

Abstract

The present investigation focused on screening of a new potent strain for laccase production and optimizing the process parameters to achieve the maximum enzymatic decolourization of textile azo dye Congo red. Seven hydrocarbonoclastic bacterial strains were selected as positive in laccase production in solid medium using 2,6 dimethoxyphenol as an enzyme activity indicator. The best enzyme producer Pseudomonas extremorientalis BU118 showed a maximum laccase activity of about 7000 U/L of wheat bran under solid-state conditions. The influence of different concentrations of dye, enzyme, salt and various incubation times on Congo red decolourization was studied using response surface methodology to find the optimum conditions required for maximum decolourization by P. extremorientalis laccase. The enzyme exhibited a remarkable colour removal capability over a wide range of dye and salt concentrations. The above results show the potential use of this bacterial laccase in the biological treatment of the textile effluent.

Keywords: Azo dye decolourization; Central composite design; Pseudomonas extremorientalis laccase; Response surface methodology; Salt tolerance

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