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Arun M, Chinnathambi A, Subramanyam K, et al. Involvement of exogenous polyamines enhances regeneration and Agrobacterium-mediated genetic transformation in half-seeds of soybean. 3 Biotech. 2016;6(2):148doi: 10.1007/s13205-016-0448-0.
Arun, M., Chinnathambi, A., Subramanyam, K., Karthik, S., Sivanandhan, G., Theboral, J., Alharbi, S. A., Kim, C. K., & Ganapathi, A. (2016). Involvement of exogenous polyamines enhances regeneration and Agrobacterium-mediated genetic transformation in half-seeds of soybean. 3 Biotech, 6(2), 148. https://doi.org/10.1007/s13205-016-0448-0
Arun, Muthukrishnan, et al. "Involvement of exogenous polyamines enhances regeneration and Agrobacterium-mediated genetic transformation in half-seeds of soybean." 3 Biotech vol. 6,2 (2016): 148. doi: https://doi.org/10.1007/s13205-016-0448-0
Arun M, Chinnathambi A, Subramanyam K, Karthik S, Sivanandhan G, Theboral J, Alharbi SA, Kim CK, Ganapathi A. Involvement of exogenous polyamines enhances regeneration and Agrobacterium-mediated genetic transformation in half-seeds of soybean. 3 Biotech. 2016 Dec;6(2):148. doi: 10.1007/s13205-016-0448-0. Epub 2016 Jun 28. PMID: 28330220; PMCID: PMC4925569.
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3 Biotech. 2016 Dec;6(2):148. doi: 10.1007/s13205-016-0448-0. Epub 2016 Jun 28.

Involvement of exogenous polyamines enhances regeneration and Agrobacterium-mediated genetic transformation in half-seeds of soybean.

3 Biotech

Muthukrishnan Arun, Arunachalam Chinnathambi, Kondeti Subramanyam, Sivabalan Karthik, Ganeshan Sivanandhan, Jeevaraj Theboral, Sulaiman Ali Alharbi, Chang Kil Kim, Andy Ganapathi

Affiliations

  1. Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620024, India.
  2. Department of Horticultural Sciences, Kyungpook National University, Daegu, 702-701, South Korea.
  3. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia.
  4. Laboratory of Biochemistry and Glycobiology, Department of Molecular Biotechnology, Ghent University, Coupure links 653, 9000, Ghent, Belgium.
  5. Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620024, India. [email protected].
  6. Bharathiar University, Coimbatore, Tamil Nadu, 641046, India. [email protected].

PMID: 28330220 PMCID: PMC4925569 DOI: 10.1007/s13205-016-0448-0

Abstract

The present work demonstrates the participation of polyamines (PAs) to improve direct regeneration and Agrobacterium-mediated transformation in soybean half-seeds. The inclusion of PAs to culture medium along with optimal plant growth regulators (PGRs) enhanced shoot induction [98.3 %; 4.44 µM N

Keywords: Half-seed; Putrescine; Soybean; Spermidine; Spermine

References

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