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Dubey G, Kollah B, Gour VK, et al. Diversity of bacteria and archaea in the rhizosphere of bioenergy crop Jatropha curcas. 3 Biotech. 2016;6(2):257doi: 10.1007/s13205-016-0546-z.
Dubey, G., Kollah, B., Gour, V. K., Shukla, A. K., & Mohanty, S. R. (2016). Diversity of bacteria and archaea in the rhizosphere of bioenergy crop Jatropha curcas. 3 Biotech, 6(2), 257. https://doi.org/10.1007/s13205-016-0546-z
Dubey, Garima, et al. "Diversity of bacteria and archaea in the rhizosphere of bioenergy crop Jatropha curcas." 3 Biotech vol. 6,2 (2016): 257. doi: https://doi.org/10.1007/s13205-016-0546-z
Dubey G, Kollah B, Gour VK, Shukla AK, Mohanty SR. Diversity of bacteria and archaea in the rhizosphere of bioenergy crop Jatropha curcas. 3 Biotech. 2016 Dec;6(2):257. doi: 10.1007/s13205-016-0546-z. Epub 2016 Dec 02. PMID: 28330329; PMCID: PMC5135702.
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3 Biotech. 2016 Dec;6(2):257. doi: 10.1007/s13205-016-0546-z. Epub 2016 Dec 02.

Diversity of bacteria and archaea in the rhizosphere of bioenergy crop Jatropha curcas.

3 Biotech

Garima Dubey, Bharati Kollah, Vijay Kumar Gour, Arvind Kumar Shukla, Santosh Ranjan Mohanty

Affiliations

  1. Indian Institute of Soil Science, Nabibagh, Bhopal, 462038, India.
  2. Department of Plant Breeding and Genetics, J.N. Agricultural University, Krishinagar, Jabalpur, 482004, India.
  3. Indian Institute of Soil Science, Nabibagh, Bhopal, 462038, India. [email protected].

PMID: 28330329 PMCID: PMC5135702 DOI: 10.1007/s13205-016-0546-z

Abstract

Plant-microbial interaction in rhizosphere plays vital role in shaping plant's growth and ecosystem function. Most of the rhizospheric microbial diversity studies are restricted to bacteria. In natural ecosystem, archaea also constitutes a major component of the microbial population. However, their diversity is less known compared to bacteria. Experiments were carried out to examine diversity of bacteria and archaea in the rhizosphere of bioenergy crop Jatropha curcas (J. curcas). Samples were collected from three locations varying widely in the soil physico-chemical properties. Diversity was estimated by terminal restriction fragment length polymorphism (TRFLP) targeting 16S rRNA gene of bacteria and archaea. Fifteen bacterial and 17 archaeal terminal restriction fragments (TRFs) were retrieved from J. curcas rhizosphere. Bacterial indicative TRFs were Actinobacteria, Firmicutes, Acidobacteria, Verrumicrobiaceae, and Chlroflexi. Major archaeal TRFs were crenarchaeota, and euryarchaeota. In case of bacteria, relative fluorescence was low for TRF160 and high for TRF51, TRF 420. Similarly, for archaea relative fluorescence of TRF 218, and TRF 282 was low and high for TRF 278, TRF468 and TRF93. Principal component analysis (PCA) of bacterial TRFs designated PC 1 with 46.83% of variation and PC2 with 31.07% variation. Archaeal TRFs designated 90.94% of variation by PC1 and 9.05% by PC2. Simpson index varied from 0.530 to 0.880 and Shannon index from 1.462 to 3.139 for bacteria. For archaea, Simpson index varied from 0.855 to 0.897 and Shannon index varied from 3.027 to 3.155. Study concluded that rhizosphere of J. curcas constituted of diverse set of both bacteria and archaea, which might have promising plant growth promoting activities.

Keywords: Archaea; Bacteria; Bioenergy crop; Diversity; Jatropha curcas; Rhizosphere

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