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Ayuso-Fernández I, Martínez AT, Ruiz-Dueñas FJ. Experimental recreation of the evolution of lignin-degrading enzymes from the Jurassic to date. Biotechnol Biofuels. 2017;10:67doi: 10.1186/s13068-017-0744-x.
Ayuso-Fernández, I., Martínez, A. T., & Ruiz-Dueñas, F. J. (2017). Experimental recreation of the evolution of lignin-degrading enzymes from the Jurassic to date. Biotechnology for biofuels, 1067. https://doi.org/10.1186/s13068-017-0744-x
Ayuso-Fernández, Iván, et al. "Experimental recreation of the evolution of lignin-degrading enzymes from the Jurassic to date." Biotechnology for biofuels vol. 10 (2017): 67. doi: https://doi.org/10.1186/s13068-017-0744-x
Ayuso-Fernández I, Martínez AT, Ruiz-Dueñas FJ. Experimental recreation of the evolution of lignin-degrading enzymes from the Jurassic to date. Biotechnol Biofuels. 2017 Mar 16;10:67. doi: 10.1186/s13068-017-0744-x. eCollection 2017. PMID: 28331543; PMCID: PMC5356311.
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Biotechnol Biofuels. 2017 Mar 16;10:67. doi: 10.1186/s13068-017-0744-x. eCollection 2017.

Experimental recreation of the evolution of lignin-degrading enzymes from the Jurassic to date.

Biotechnology for biofuels

Iván Ayuso-Fernández, Angel T Martínez, Francisco J Ruiz-Dueñas

Affiliations

  1. IPSBB unit, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.

PMID: 28331543 PMCID: PMC5356311 DOI: 10.1186/s13068-017-0744-x

Abstract

BACKGROUND: Floudas et al. (

RESULTS: With this purpose, we analyzed the evolutionary pathway leading to the most efficient lignin-degrading peroxidases characterizing Polyporales species. After sequence reconstruction from 113 genes of ten sequenced genomes, the main enzyme intermediates were resurrected and characterized. Biochemical changes were analyzed together with predicted sequences and structures, to understand how these enzymes acquired the ability to degrade lignin and how this ability changed with time. The most probable first peroxidase in Polyporales would be a manganese peroxidase (Mn

CONCLUSIONS: We show how the change in peroxidase catalytic activities meant an evolutionary exploration for more efficient ways of lignin degradation by fungi, a key step for carbon recycling in land ecosystems. The study provides ancestral enzymes with a potential biotechnological interest for the sustainable production of fuels and chemicals in a biomass-based economy.

Keywords: Ancestral sequence reconstruction; Catalytic properties; Fungal evolution; Fungal genomes; Lignin biodegradation resurrected enzymes; Ligninolytic peroxidases; pH stability

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