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Mirshamsi MR, Omranipour R, Vazirizadeh A, et al. Persian Gulf Jellyfish (Cassiopea andromeda) Venom Fractions Induce Selective Injury and Cytochrome C Release in Mitochondria Obtained from Breast Adenocarcinoma Patients. Asian Pac J Cancer Prev. 2017;18(1):277-286doi: 10.22034/APJCP.2017.18.1.277.
Mirshamsi, M. R., Omranipour, R., Vazirizadeh, A., Fakhri, A., Zangeneh, F., Mohebbi, G. H., Seyedian, R., & Pourahmad, J. (2017). Persian Gulf Jellyfish (Cassiopea andromeda) Venom Fractions Induce Selective Injury and Cytochrome C Release in Mitochondria Obtained from Breast Adenocarcinoma Patients. Asian Pacific journal of cancer prevention : APJCP, 18(1), 277-286. https://doi.org/10.22034/APJCP.2017.18.1.277
Mirshamsi, Mohammad Reza, et al. "Persian Gulf Jellyfish (Cassiopea andromeda) Venom Fractions Induce Selective Injury and Cytochrome C Release in Mitochondria Obtained from Breast Adenocarcinoma Patients." Asian Pacific journal of cancer prevention : APJCP vol. 18,1 (2017): 277-286. doi: https://doi.org/10.22034/APJCP.2017.18.1.277
Mirshamsi MR, Omranipour R, Vazirizadeh A, Fakhri A, Zangeneh F, Mohebbi GH, Seyedian R, Pourahmad J. Persian Gulf Jellyfish (Cassiopea andromeda) Venom Fractions Induce Selective Injury and Cytochrome C Release in Mitochondria Obtained from Breast Adenocarcinoma Patients. Asian Pac J Cancer Prev. 2017 Jan 01;18(1):277-286. doi: 10.22034/APJCP.2017.18.1.277. PMID: 28240847; PMCID: PMC5563113.
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Asian Pac J Cancer Prev. 2017 Jan 01;18(1):277-286. doi: 10.22034/APJCP.2017.18.1.277.

Persian Gulf Jellyfish (Cassiopea andromeda) Venom Fractions Induce Selective Injury and Cytochrome C Release in Mitochondria Obtained from Breast Adenocarcinoma Patients.

Asian Pacific journal of cancer prevention : APJCP

Mohammad Reza Mirshamsi, Ramesh Omranipour, Amir Vazirizadeh, Amir Fakhri, Fatemeh Zangeneh, Gholam Hussain Mohebbi, Ramin Seyedian, Jalal Pourahmad

Affiliations

  1. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Email: [email protected]

PMID: 28240847 PMCID: PMC5563113 DOI: 10.22034/APJCP.2017.18.1.277

Abstract

Objective: This study was conducted to investigate whether fractions of jellyfish Cassiope andromeda venom, could selectively induce toxicity on mitochondria isolated from cancer tissue of patients with breast adenocarcinomas. Methods: Firstly, we extracted two fractions, (f1 and f2) from crude jellyfish venom by gel filtration on Sephadex G-200.Then different dilutions of these extracted fractions were applied to mitochondria isolated from human breast tumoral- and extra-tumoral tissues. Parameters of mitochondrial toxicity including generation of reactive oxygen species (ROS), mitochondrial membrane potential (MMP) collapse, swelling, cytochrome c release, activation of caspase3 and apoptosis were then assayed. Result: Our results demonstrate that fraction 2 of Cassiopea andromeda crude venom significantly (P<0.05) decreased mitochondrial succinate dehydrogenase activity, increased mitochondrial ROS production, induced mitochondrial swelling, MMP collapse and cytochrome c release, activated caspase3 and induced apoptosis only in tumoral mitochondria, and not in mitochondria obtained from extra-tumoral tissue (P<0.05). Conclusion: In conclusion this study suggested that fraction 2 of Cassiopea andromeda crude venom selectively induces ROS mediated cytotoxicity by directly targeting mitochondria isolated from human breast tumor tissue.

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Keywords: Cassiopea andromeda venom; gel filtration; selective toxicity; Isolated mitochondria; Apoptosis

References

  1. Cancer Res. 2005 Mar 1;65(5):1984-93 - PubMed
  2. Mol Aspects Med. 2010 Feb;31(1):29-59 - PubMed
  3. Toxicon. 1990;28(7):821-35 - PubMed
  4. Mar Drugs. 2013 May 22;11(5):1728-62 - PubMed
  5. Biochim Biophys Acta. 2003 Sep 30;1606(1-3):137-46 - PubMed
  6. Drug Resist Updat. 2004 Apr;7(2):97-110 - PubMed
  7. Int J Biochem Cell Biol. 2000 Feb;32(2):157-70 - PubMed
  8. Lipids Health Dis. 2011 Dec 09;10:232 - PubMed
  9. Nat Protoc. 2006;1(3):1458-61 - PubMed
  10. Bioorg Med Chem Lett. 2005 Nov 15;15(22):4949-52 - PubMed
  11. Mitochondrion. 2010 Nov;10(6):670-81 - PubMed
  12. Biochim Biophys Acta. 2012 Dec;1820(12):1940-50 - PubMed
  13. Int J Cancer. 2015 Mar 1;136(5):E359-86 - PubMed
  14. Nat Rev Drug Discov. 2010 Jun;9(6):447-64 - PubMed
  15. Curr Pharm Des. 2011 Dec 1;17(36):4034-46 - PubMed
  16. Bioorg Med Chem Lett. 2005 May 16;15(10):2659-64 - PubMed
  17. Chem Biol Interact. 2012 Aug 30;199(2):129-36 - PubMed
  18. Comp Biochem Physiol C Toxicol Pharmacol. 2009 Jul;150(1):85-90 - PubMed
  19. Toxicon. 2002 Apr;40(4):441-6 - PubMed
  20. Lipids Health Dis. 2012 Jun 28;11:84 - PubMed
  21. Virchows Arch. 2000 Aug;437(2):107-15 - PubMed
  22. Arch Immunol Ther Exp (Warsz). 2013 Feb;61(1):43-58 - PubMed
  23. Biomed Res Int. 2014;2014:892170 - PubMed
  24. Comp Biochem Physiol C Toxicol Pharmacol. 2016 Feb;180:31-9 - PubMed
  25. Toxicon. 1992 Jul;30(7):794-6 - PubMed
  26. Mitochondrion. 2002 Dec;2(3):199-210 - PubMed
  27. Biochem Biophys Res Commun. 1985 Jul 31;130(2):515-23 - PubMed
  28. Toxicol Lett. 2005 Feb 15;155(2):219-26 - PubMed
  29. Toxicon. 1998 Aug;36(8):1075-85 - PubMed
  30. Mol Cell Biochem. 2010 May;338(1-2):69-76 - PubMed
  31. Toxicol In Vitro. 2012 Aug;26(5):694-9 - PubMed
  32. Food Chem Toxicol. 2014 May;67:193-200 - PubMed
  33. Toxicon. 2016 Feb;110:74-8 - PubMed
  34. Toxicon. 2001 May;39(5):693-702 - PubMed
  35. Comp Biochem Physiol C Toxicol Pharmacol. 2004 Dec;139(4):295-301 - PubMed
  36. Toxicol Ind Health. 2012 Mar;28(2):186-92 - PubMed
  37. Biochem Biophys Res Commun. 2006 Sep 22;348(2):514-23 - PubMed
  38. Br J Cancer. 1998 Dec;78(11):1421-9 - PubMed
  39. Redox Biol. 2015 Dec;6:461-471 - PubMed
  40. Metallomics. 2013 Feb;5(2):152-66 - PubMed
  41. Cell Calcium. 2011 Sep;50(3):211-21 - PubMed
  42. Toxicol Lett. 2014 May 16;227(1):65-73 - PubMed
  43. Free Radic Biol Med. 2010 Mar 15;48(6):749-62 - PubMed
  44. FEBS Lett. 2001 Apr 20;495(1-2):12-5 - PubMed

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