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Moein S, Javanmard SH, Abedi M, et al. Identification of Appropriate Housekeeping Genes for Gene Expression Analysis in Long-term Hypoxia-treated Kidney Cells. Adv Biomed Res. 2017;6:15doi: 10.4103/2277-9175.200790.
Moein, S., Javanmard, S. H., Abedi, M., Izadpanahi, M. H., & Gheisari, Y. (2017). Identification of Appropriate Housekeeping Genes for Gene Expression Analysis in Long-term Hypoxia-treated Kidney Cells. Advanced biomedical research, 615. https://doi.org/10.4103/2277-9175.200790
Moein, Shiva, et al. "Identification of Appropriate Housekeeping Genes for Gene Expression Analysis in Long-term Hypoxia-treated Kidney Cells." Advanced biomedical research vol. 6 (2017): 15. doi: https://doi.org/10.4103/2277-9175.200790
Moein S, Javanmard SH, Abedi M, Izadpanahi MH, Gheisari Y. Identification of Appropriate Housekeeping Genes for Gene Expression Analysis in Long-term Hypoxia-treated Kidney Cells. Adv Biomed Res. 2017 Feb 22;6:15. doi: 10.4103/2277-9175.200790. eCollection 2017. PMID: 28299307; PMCID: PMC5343613.
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Adv Biomed Res. 2017 Feb 22;6:15. doi: 10.4103/2277-9175.200790. eCollection 2017.

Identification of Appropriate Housekeeping Genes for Gene Expression Analysis in Long-term Hypoxia-treated Kidney Cells.

Advanced biomedical research

Shiva Moein, Shaghayegh Haghjooy Javanmard, Maryam Abedi, Mohammad Hosein Izadpanahi, Yousof Gheisari

Affiliations

  1. Department of Genetics and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran.
  2. Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
  3. Department of Urology, Isfahan University of Medical Sciences, Isfahan, Iran.
  4. Department of Genetics and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran; Regenerative Medicine Lab, Isfahan Kidney Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

PMID: 28299307 PMCID: PMC5343613 DOI: 10.4103/2277-9175.200790

Abstract

BACKGROUND: Selection of stably expressing housekeeping genes (HKGs) is a crucial step in gene expression analysis. However, there are no universal HKGs for all experiments, and they should be determined by each biologic condition. The aim of this study was to detect appropriate HKGs for kidney cells cultured in long-term hypoxia.

MATERIALS AND METHODS: Based on a screening step using a microarray data available from gene expression omnibus database, a set of candidate HKGs were chosen to be assessed in human kidney cells cultured in hypoxic or normoxic conditions for about 2 weeks in a time course manner. The stability of gene expression was assessed by refFinder, a web-based tool that integrates four computational programs (geNorm, Normfinder, BestKeeper, and the comparative ΔΔCt method).

RESULTS: GAPDH and ACTB were the most stable genes in hypoxia treated cells whereas, B2M and ACTB were the best HKGs in cells cultured in normoxia. When both hypoxia and normoxia treated cells from all time points were evaluated together, GAPDH and ACTB equally showed the most stability.

CONCLUSION: As in relative quantification of real-time polymerase chain reaction data, the same HKGs should be selected for all groups, we believe that GAPDH and ACTB are suitable HKGs for studies on the effect of hypoxia on cultured kidney cells.

Keywords: Gene expression analysis; housekeeping gene; hypoxia; kidney

Conflict of interest statement

There are no conflicts of interest.

References

  1. G3 (Bethesda). 2014 Sep 05;4(11):2159-65 - PubMed
  2. Mol Med. 2008 Jul-Aug;14 (7-8):502-16 - PubMed
  3. Kidney Int. 2008 Oct;74(7):867-72 - PubMed
  4. Int J Biochem Mol Biol. 2012;3(2):165-78 - PubMed
  5. Am J Physiol Lung Cell Mol Physiol. 2008 Aug;295(2):L272-84 - PubMed
  6. Lab Invest. 2005 Jan;85(1):154-9 - PubMed
  7. Mol Biol Rep. 2012 Apr;39(4):4857-67 - PubMed
  8. Cell Cycle. 2008 May 1;7(9):1128-32 - PubMed
  9. Nat Protoc. 2006;1(3):1559-82 - PubMed
  10. Br J Cancer. 2010 Aug 10;103(4):590; author reply 591-2 - PubMed
  11. BMC Mol Biol. 2009 Oct 09;10:94 - PubMed
  12. Kidney Int Suppl. 2000 Apr;75:S22-6 - PubMed
  13. Nat Rev Drug Discov. 2009 Feb;8(2):139-52 - PubMed
  14. Br J Cancer. 2010 Mar 16;102(6):1037-43 - PubMed
  15. J Biochem Biophys Methods. 2000 Nov 20;46(1-2):69-81 - PubMed

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