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Heyde KC, Scott FY, Paek SH, et al. Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials. J Vis Exp. 2017;(121)doi: 10.3791/55300.
Heyde, K. C., Scott, F. Y., Paek, S. H., Zhang, R., & Ruder, W. C. (2017). Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials. Journal of visualized experiments : JoVE, (121), . https://doi.org/10.3791/55300
Heyde, Keith C, et al. "Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials." Journal of visualized experiments : JoVE vol. ,121 (2017). doi: https://doi.org/10.3791/55300
Heyde KC, Scott FY, Paek SH, Zhang R, Ruder WC. Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials. J Vis Exp. 2017 Mar 09;(121). doi: 10.3791/55300. PMID: 28362372; PMCID: PMC5409335.
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J Vis Exp. 2017 Mar 09;(121). doi: 10.3791/55300.

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials.

Journal of visualized experiments : JoVE

Keith C Heyde, Felicia Y Scott, Sung-Ho Paek, Ruihua Zhang, Warren C Ruder

Affiliations

  1. Department of Mechanical Engineering, Carnegie Mellon University; Engineering Science and Mechanics Program, Virginia Polytechnic Institute and State University.
  2. Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University.
  3. Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University; Department of Bioengineering, University of Pittsburgh; [email protected].

PMID: 28362372 PMCID: PMC5409335 DOI: 10.3791/55300

Abstract

We have developed an abiotic-biotic interface that allows engineered cells to control the material properties of a functionalized surface. This system is made by creating two modules: a synthetically engineered strain of E. coli cells and a functionalized material interface. Within this paper, we detail a protocol for genetically engineering selected behaviors within a strain of E. coli using molecular cloning strategies. Once developed, this strain produces elevated levels of biotin when exposed to a chemical inducer. Additionally, we detail protocols for creating two different functionalized surfaces, each of which is able to respond to cell-synthesized biotin. Taken together, we present a methodology for creating a linked, abiotic-biotic system that allows engineered cells to control material composition and assembly on nonliving substrates.

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