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Espinoza-Sánchez NA, Chimal-Ramírez GK, Mantilla A, et al. IL-1β, IL-8, and Matrix Metalloproteinases-1, -2, and -10 Are Enriched upon Monocyte-Breast Cancer Cell Cocultivation in a Matrigel-Based Three-Dimensional System. Front Immunol. 2017;8:205doi: 10.3389/fimmu.2017.00205.
Espinoza-Sánchez, N. A., Chimal-Ramírez, G. K., Mantilla, A., & Fuentes-Pananá, E. M. (2017). IL-1β, IL-8, and Matrix Metalloproteinases-1, -2, and -10 Are Enriched upon Monocyte-Breast Cancer Cell Cocultivation in a Matrigel-Based Three-Dimensional System. Frontiers in immunology, 8205. https://doi.org/10.3389/fimmu.2017.00205
Espinoza-Sánchez, Nancy Adriana, et al. "IL-1β, IL-8, and Matrix Metalloproteinases-1, -2, and -10 Are Enriched upon Monocyte-Breast Cancer Cell Cocultivation in a Matrigel-Based Three-Dimensional System." Frontiers in immunology vol. 8 (2017): 205. doi: https://doi.org/10.3389/fimmu.2017.00205
Espinoza-Sánchez NA, Chimal-Ramírez GK, Mantilla A, Fuentes-Pananá EM. IL-1β, IL-8, and Matrix Metalloproteinases-1, -2, and -10 Are Enriched upon Monocyte-Breast Cancer Cell Cocultivation in a Matrigel-Based Three-Dimensional System. Front Immunol. 2017 Mar 08;8:205. doi: 10.3389/fimmu.2017.00205. eCollection 2017. PMID: 28337194; PMCID: PMC5340783.
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Front Immunol. 2017 Mar 08;8:205. doi: 10.3389/fimmu.2017.00205. eCollection 2017.

IL-1β, IL-8, and Matrix Metalloproteinases-1, -2, and -10 Are Enriched upon Monocyte-Breast Cancer Cell Cocultivation in a Matrigel-Based Three-Dimensional System.

Frontiers in immunology

Nancy Adriana Espinoza-Sánchez, Gloria Karina Chimal-Ramírez, Alejandra Mantilla, Ezequiel Moisés Fuentes-Pananá

Affiliations

  1. Unidad de Investigación en Virología y Cáncer, Hospital Infantil de México Federico Gómez, Ciudad de México, México; Programa de Doctorado en Ciencias Biomédicas, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México, México.
  2. Unidad de Investigación en Virología y Cáncer, Hospital Infantil de México Federico Gómez , Ciudad de México , México.
  3. Departamento de Patología, Hospital de Oncología, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social , Ciudad de México , México.

PMID: 28337194 PMCID: PMC5340783 DOI: 10.3389/fimmu.2017.00205

Abstract

Breast cancer remains the first cancer-related cause of death in women worldwide, particularly in developing countries in which most cases are diagnosed in late stages. Although most cancer studies are based in the genetic or epigenetic changes of the tumor cells, immune cells within the tumor stroma often cooperate with cancer progression. Particularly, monocytes are attracted to the tumor primary site in which they are differentiated into tumor-associated macrophages that facilitate tumor cell invasion and metastasis. In this study, we used three-dimensional cultures to form acini-like structures to analyze the inflammatory secretion profile of tumor cells individually or in co-culture with monocytes. Breast cancer cell lines and primary isolates from eight Mexican patients with breast cancer were used. We found high levels of RANTES/CCL5, MCP-1/CCL2, and G-CSF in the breast cancer individual cultures, supporting an important recruitment capacity of monocytes, but also of neutrophils. The co-cultures of the tumor cells and monocytes were significantly enriched with the potent pro-inflammatory cytokines interleukin (IL)-1β and IL-8, known to support malignant progression. We also found that the interaction of tumor cells with monocytes promoted high levels of matrix metalloproteinases (MMP)-1, MMP-2, and MMP-10. Our study supports that a key event for malignant progression is the recruitment of different immune cell populations, which help to sustain and enhance a chronic inflammatory microenvironment that highly favors tumor malignancy.

Keywords: IL-1β; IL-8; MMPs; breast cancer; inflammation; matrigel-based 3D culture; monocytes

References

  1. Scientifica (Cairo). 2014;2014:970315 - PubMed
  2. Angiogenesis. 2005;8(1):63-71 - PubMed
  3. Cancer Invest. 2000;18(4):293-302 - PubMed
  4. Clin Cancer Res. 2007 Jan 1;13(1):215-22 - PubMed
  5. Breast Cancer Res. 2009;11(2):R21 - PubMed
  6. J Cancer Res Ther. 2014 Jul-Sep;10(3):506-11 - PubMed
  7. Prostate Cancer Prostatic Dis. 2003;6(3):217-22 - PubMed
  8. Breast Cancer Res Treat. 2013 Nov;142(2):237-55 - PubMed
  9. J Immunol. 2009 Mar 1;182(5):2786-94 - PubMed
  10. Nat Rev Immunol. 2003 Feb;3(2):133-46 - PubMed
  11. BMC Cancer. 2014 May 03;14:310 - PubMed
  12. Cell. 2010 Mar 19;140(6):883-99 - PubMed
  13. J Immunol. 1999 Sep 1;163(5):2561-7 - PubMed
  14. Int J Oncol. 2017 Feb;50(2):432-440 - PubMed
  15. Cell. 2003 Jul 11;114(1):33-45 - PubMed
  16. Expert Opin Ther Targets. 2013 Nov;17(11):1235-41 - PubMed
  17. Cancer Immunol Res. 2014 Aug;2(8):765-76 - PubMed
  18. Cancer Res. 1990 Feb 1;50(3):717-21 - PubMed
  19. Crit Rev Immunol. 2001;21(5):399-425 - PubMed
  20. PLoS One. 2011;6(11):e27690 - PubMed
  21. Br J Cancer. 1991 Feb;63(2):287-92 - PubMed
  22. Int J Biochem Cell Biol. 2005 Feb;37(2):283-8 - PubMed
  23. BMC Cancer. 2012 Dec 07;12:583 - PubMed
  24. Phytother Res. 2014 Nov;28(11):1654-60 - PubMed
  25. Cancer Epidemiol Biomarkers Prev. 2012 Dec;21(12):2149-58 - PubMed
  26. Ann Oncol. 2007 Feb;18(2):226-32 - PubMed
  27. Melanoma Res. 2007 Aug;17(4):205-13 - PubMed
  28. J Clin Invest. 2013 Aug;123(8):3383-94 - PubMed
  29. Clin Cancer Res. 2008 Nov 1;14(21):6735-41 - PubMed
  30. Clin Cancer Res. 2000 May;6(5):2104-19 - PubMed
  31. Cancer Res. 2015 Jun 15;75(12):2566-79 - PubMed
  32. Cell. 2011 Mar 4;144(5):646-74 - PubMed
  33. Leuk Res. 2011 Feb;35(2):226-36 - PubMed
  34. Proc Natl Acad Sci U S A. 2010 Dec 14;107(50):21248-55 - PubMed
  35. Technol Cancer Res Treat. 2013 Jun;12(3):259-67 - PubMed
  36. Experientia. 1991 Jan 15;47(1):22-31 - PubMed
  37. Breast Cancer Res Treat. 2004 Feb;83(3):249-89 - PubMed
  38. Cytokine Growth Factor Rev. 2014 Aug;25(4):355-67 - PubMed
  39. J Oncol. 2013;2013:835956 - PubMed
  40. J Immunol Res. 2016;2016:6031486 - PubMed
  41. Genes Dev. 2009 Aug 15;23 (16):1882-94 - PubMed
  42. Breast Cancer Res. 2003;5(1):31-6 - PubMed
  43. J Interferon Cytokine Res. 2013 Oct;33(10):563-70 - PubMed
  44. Biomed Res Int. 2013;2013:279505 - PubMed
  45. Oncoimmunology. 2013 Jul 1;2(7):e25474 - PubMed
  46. J Leukoc Biol. 2009 Nov;86(5):1065-73 - PubMed
  47. Cancer Cell. 2009 Sep 8;16(3):183-94 - PubMed
  48. J Clin Oncol. 2003 Jun 15;21(12):2342-8 - PubMed
  49. Neoplasia. 2004 Nov-Dec;6(6):777-85 - PubMed
  50. Cell Res. 2006 Feb;16(2):126-33 - PubMed
  51. Carcinogenesis. 2012 Jul;33(7):1302-9 - PubMed
  52. J Urol. 2013 Dec;190(6):2257-62 - PubMed
  53. J Surg Oncol. 2015 May;111(6):752-9 - PubMed
  54. Nat Med. 1996 Apr;2(4):461-2 - PubMed
  55. Breast Cancer Res Treat. 2014 Nov;148(2):269-77 - PubMed
  56. Sci Rep. 2014 Aug 01;4:5911 - PubMed
  57. Nat Rev Cancer. 2004 Jan;4(1):71-8 - PubMed
  58. BMC Cancer. 2012 Jul 23;12:306 - PubMed
  59. Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2645-50 - PubMed
  60. Cancer Biol Ther. 2015;16(8):1220-30 - PubMed
  61. Cancer Treat Rev. 2012 Oct;38(6):689-97 - PubMed
  62. J Immunol. 2005 Jul 1;175(1):342-9 - PubMed
  63. Trends Immunol. 2013 Aug;34(8):398-409 - PubMed
  64. Nat Med. 2005 May;11(5):481-3 - PubMed
  65. Mol Neurobiol. 2015 Aug;52(1):303-17 - PubMed
  66. PLoS One. 2010 Jan 13;5(1):e8668 - PubMed
  67. Mol Cancer. 2012 Nov 23;11:87 - PubMed
  68. Clin Cancer Res. 2004 Nov 15;10(22):7621-8 - PubMed
  69. Clin Cancer Res. 2001 Aug;7(8):2396-404 - PubMed
  70. Cancer Immunol Immunother. 2014 May;63(5):419-35 - PubMed
  71. Immunol Rev. 2014 Nov;262(1):36-55 - PubMed
  72. Blood. 1997 Aug 15;90(4):1649-55 - PubMed
  73. Cell. 2006 Jan 27;124(2):263-6 - PubMed
  74. Cell. 2010 Apr 2;141(1):39-51 - PubMed
  75. Front Biosci. 2005 Jan 01;10:853-65 - PubMed
  76. Neoplasia. 2013 Dec;15(12 ):1330-46 - PubMed
  77. J Exp Med. 2001 Mar 19;193(6):727-40 - PubMed
  78. PLoS One. 2012;7(10):e47469 - PubMed
  79. Mol Cancer. 2012 Oct 21;11:79 - PubMed
  80. BMC Cancer. 2011 Apr 12;11:130 - PubMed
  81. Cancer Epidemiol Biomarkers Prev. 2008 May;17(5):1127-35 - PubMed
  82. Eur J Cell Biol. 1989 Apr;48(2):282-93 - PubMed
  83. J Transl Med. 2006 Nov 10;4:48 - PubMed
  84. Cancer Res. 2010 Aug 1;70(15):6139-49 - PubMed
  85. Oncologist. 2001;6(1):34-55 - PubMed

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