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Hizay A, Seitz M, Grosheva M, et al. FGF-2 is required to prevent astrogliosis in the facial nucleus after facial nerve injury and mechanical stimulation of denervated vibrissal muscles. J Biomed Res. 2016;30(2):142-148doi: 10.7555/JBR.30.20140042.
Hizay, A., Seitz, M., Grosheva, M., Sinis, N., Kaya, Y., Bendella, H., Sarikcioglu, L., Dunlop, S. A., & Angelov, D. N. (2016). FGF-2 is required to prevent astrogliosis in the facial nucleus after facial nerve injury and mechanical stimulation of denervated vibrissal muscles. Journal of biomedical research, 30(2), 142-148. https://doi.org/10.7555/JBR.30.20140042
Hizay, Arzu, et al. "FGF-2 is required to prevent astrogliosis in the facial nucleus after facial nerve injury and mechanical stimulation of denervated vibrissal muscles." Journal of biomedical research vol. 30,2 (2016): 142-148. doi: https://doi.org/10.7555/JBR.30.20140042
Hizay A, Seitz M, Grosheva M, Sinis N, Kaya Y, Bendella H, Sarikcioglu L, Dunlop SA, Angelov DN. FGF-2 is required to prevent astrogliosis in the facial nucleus after facial nerve injury and mechanical stimulation of denervated vibrissal muscles. J Biomed Res. 2016 Mar;30(2):142-148. doi: 10.7555/JBR.30.20140042. Epub 2016 Mar 20. PMID: 28276669; PMCID: PMC4820891.
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J Biomed Res. 2016 Mar;30(2):142-148. doi: 10.7555/JBR.30.20140042. Epub 2016 Mar 20.

FGF-2 is required to prevent astrogliosis in the facial nucleus after facial nerve injury and mechanical stimulation of denervated vibrissal muscles.

Journal of biomedical research

Arzu Hizay, Mark Seitz, Maria Grosheva, Nektarios Sinis, Yasemin Kaya, Habib Bendella, Levent Sarikcioglu, Sarah A Dunlop, Doychin N Angelov

Affiliations

  1. Department of Anatomy, Akdeniz University, Faculty of Medicine, Dumlupinar Bulvari 07058 Kampus, Antalya, Turkey.
  2. Department of Anatomy I, University of Cologne, Joseph-Stelzmann-Strasse 9, D-50924 Cologne, FR Germany.
  3. Department of Oto-Rhino-Laryngology, University Hospital Cologne, Joseph-Stelzmann-Strasse 9, D-50924 Cologne, FR Germany.
  4. Depeartment of Plastic-, Hand- and Reconstructive Microsurgery, Berlin, FR 12249, Germany.
  5. Department of Neurosurgery, Hospital Merheim, University of Witten-Herdecke, Ostmerheimer Straße 200, 51109 Cologne, Germany.
  6. Experimental and Regenerative Neurosciences, School of Animal Biology, University of Western Australia (M092) 35 Stirling Highway, CRAWLEY WA 6009, Australia.
  7. Department of Anatomy I, University of Cologne, Joseph-Stelzmann-Strasse 9, D-50924 Cologne, FR Germany; [email protected].

PMID: 28276669 PMCID: PMC4820891 DOI: 10.7555/JBR.30.20140042

Abstract

Recently, we have shown that manual stimulation of paralyzed vibrissal muscles after facial-facial anastomosis reduced the poly-innervation of neuromuscular junctions and restored vibrissal whisking. Using gene knock outs, we found a differential dependence of manual stimulation effects on growth factors. Thus, insulin-like growth factor-1 and brain-derived neurotrophic factor are required to underpin manual stimulation-mediated improvements, whereas FGF-2 is not. The lack of dependence on FGF-2 in mediating these peripheral effects prompted us to look centrally, i.e. within the facial nucleus where increased astrogliosis after facial-facial anastomosis follows "synaptic stripping". We measured the intensity of Cy3-fluorescence after immunostaining for glial fibrillary acidic protein (GFAP) as an indirect indicator of synaptic coverage of axotomized neurons in the facial nucleus of mice lacking FGF-2

© 2016 by the Journal of Biomedical Research. All rights reserved.

Keywords: FGF-2; astrogliosis; axotomy; facial nerve; polyinnervation; whisking function

References

  1. Eur Arch Otorhinolaryngol. 1997;254(2):81-5 - PubMed
  2. Adv Anat Embryol Cell Biol. 2005;180:1-130 - PubMed
  3. Neuroscience. 2010 Sep 29;170(1):372-80 - PubMed
  4. Mol Cell Neurosci. 2001 Jan;17(1):17-30 - PubMed
  5. Glia. 2014 Aug;62(8):1328-44 - PubMed
  6. J Neurocytol. 1986 Jun;15(3):363-73 - PubMed
  7. EMBO J. 1998 Aug 3;17(15):4213-25 - PubMed
  8. Dev Neurosci. 2004 Mar-Aug;26(2-4):181-96 - PubMed
  9. Acta Neuropathol. 1993;86(2):179-81 - PubMed
  10. Glia. 2011 May;59(5):708-19 - PubMed
  11. J Comp Neurol. 2005 Oct 3;490(4):427-41 - PubMed
  12. J Neurosci Res. 1999 May 15;56(4):334-48 - PubMed
  13. J Neurotrauma. 2004 Oct;21(10):1468-78 - PubMed
  14. Eur Arch Otorhinolaryngol. 1996;253(8):475-80 - PubMed
  15. J Neurotrauma. 1999 May;16(5):347-56 - PubMed
  16. Neuroscience. 2011 May 19;182:241-7 - PubMed
  17. Brain Res. 1981 Aug 3;217(2):368-74 - PubMed
  18. Neurobiol Dis. 2007 Apr;26(1):229-42 - PubMed
  19. J Neurocytol. 1997 Aug;26(8):511-28 - PubMed
  20. Muscle Nerve. 2009 Feb;39(2):197-205 - PubMed
  21. J Cell Biol. 1990 Apr;110(4):1353-60 - PubMed
  22. Exp Neurol. 2010 Apr;222(2):226-34 - PubMed
  23. Nature. 1979 Dec 13;282(5740):724-6 - PubMed

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