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Benson J, Oberlander H, Koreeda M, et al. Isolation of a fat body factor which stimulates evagination ofGalleria mellonella wing disksin vitro. Wilhelm Roux Arch Entwickl Mech Org. 1974;175(4):327-338doi: 10.1007/BF00574897.
Benson, J., Oberlander, H., Koreeda, M., & Nakanishi, K. (1974). Isolation of a fat body factor which stimulates evagination ofGalleria mellonella wing disksin vitro. Wilhelm Roux' Archiv fur Entwicklungsmechanik der Organismen, 175(4), 327-338. https://doi.org/10.1007/BF00574897
Benson, J, et al. "Isolation of a fat body factor which stimulates evagination ofGalleria mellonella wing disksin vitro." Wilhelm Roux' Archiv fur Entwicklungsmechanik der Organismen vol. 175,4 (1974): 327-338. doi: https://doi.org/10.1007/BF00574897
Benson J, Oberlander H, Koreeda M, Nakanishi K. Isolation of a fat body factor which stimulates evagination ofGalleria mellonella wing disksin vitro. Wilhelm Roux Arch Entwickl Mech Org. 1974 Dec;175(4):327-338. doi: 10.1007/BF00574897. PMID: 28304713.
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Wilhelm Roux Arch Entwickl Mech Org. 1974 Dec;175(4):327-338. doi: 10.1007/BF00574897.

Isolation of a fat body factor which stimulates evagination ofGalleria mellonella wing disksin vitro.

Wilhelm Roux' Archiv fur Entwicklungsmechanik der Organismen

J Benson, H Oberlander, Mosato Koreeda, Koji Nakanishi

Affiliations

  1. Department of Biology, Brandeis University, Waltham, Massachusetts.
  2. Department of Chemistry, Columbia University, New York, N.Y.

PMID: 28304713 DOI: 10.1007/BF00574897

Abstract

In previous papers we have reported that wing disks ofGalleria mellonella complete tracheole migration and evagination when they are cultured in a chemically defined medium containing alpha-ecdysone. The presence of larval fat body in the culture medium greatly improved the response of the disks. We have isolated this fat body factor from culture medium. It is dialyzable, heat stable (100° C, 5 min), and insensitive to pronase digestion. The fat body factor, recovered from thin layer plates, stimulated evagination in the absence of alpha-ecdysone. Furthermore, radiochromatography studies with tritiated alpha-ecdysone and spectral analysis of the factor indicated that it was neither a metabolite of alpha-ecdysone nor an ecdysone-like molecule.

References

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  6. Annu Rev Entomol. 1974;19:97-115 - PubMed

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