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Wilhelm Roux Arch Entwickl Mech Org. 1974 Jun;174(2):117-132. doi: 10.1007/BF00573625.

[Morphological and biochemical characterization of the developmental stages of fertilized eggs inSphaerechinus granularis Lam : II. DNA content, DNA polymerase activity and DNase activity].

Wilhelm Roux' Archiv fur Entwicklungsmechanik der Organismen

[Article in German]
Werner E G Müller, Hans -J Breter, Gertrud Zahn, Rudolf K Zahn

Affiliations

  1. Physiologisch-Chemisches Institut der Johannes Gutenberg-Universität, Mainz, BRD.
  2. Laboratory for Marine Molecular Biology, Institute Ruder Boškovi?, Zagreb-Rovinj, Jugoslawien.

PMID: 28305042 DOI: 10.1007/BF00573625

Abstract

The investigations were performed with the eggs of the sea urchin speciesSphaerechinus granularis Lam. They were kept at 22° C under continuous aeration for up to 45 hours with stirring to compensate for sedimentation. 1. The change in DNA content, 2. the change in DNA dependent DNA polymerase activity, and 3. the change in DNase activity with time have been evaluated. 1. DNA Content of Embryos. The DNA content of the embryo development was determined by two different methods. Before and immediately after fertilization DNA content has been found to be 1.7±0.5·10

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