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Lynn DE, Oberlander H. The effect of cytoskeletal disrupting agents on the morphological response of a clonedManduca sexta cell line to 20-hydroxy-ecdysone. Wilehm Roux Arch Dev Biol. 1981;190(3):150-155doi: 10.1007/BF00867801.
Lynn, D. E., & Oberlander, H. (1981). The effect of cytoskeletal disrupting agents on the morphological response of a clonedManduca sexta cell line to 20-hydroxy-ecdysone. Wilhelm Roux's archives of developmental biology, 190(3), 150-155. https://doi.org/10.1007/BF00867801
Lynn, Dwight E, and Oberlander, Herbert. "The effect of cytoskeletal disrupting agents on the morphological response of a clonedManduca sexta cell line to 20-hydroxy-ecdysone." Wilhelm Roux's archives of developmental biology vol. 190,3 (1981): 150-155. doi: https://doi.org/10.1007/BF00867801
Lynn DE, Oberlander H. The effect of cytoskeletal disrupting agents on the morphological response of a clonedManduca sexta cell line to 20-hydroxy-ecdysone. Wilehm Roux Arch Dev Biol. 1981 May;190(3):150-155. doi: 10.1007/BF00867801. PMID: 28305165.
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Wilehm Roux Arch Dev Biol. 1981 May;190(3):150-155. doi: 10.1007/BF00867801.

The effect of cytoskeletal disrupting agents on the morphological response of a clonedManduca sexta cell line to 20-hydroxy-ecdysone.

Wilhelm Roux's archives of developmental biology

Dwight E Lynn, Herbert Oberlander

Affiliations

  1. Department of Entomology and Nematology, University of Florida, USA.
  2. Insect Attractants, Behavior, and Basic Biology Research Laboratory, Agricultural Research, Science and Education Administration, USDA, 32604, Gainesville, Florida, USA.

PMID: 28305165 DOI: 10.1007/BF00867801

Abstract

The embryonicManduca sexta cell line, MRRL-CH1 was cloned by dilution and single cell plating into conditioned medium, resulting in 6 cell clones with differing responses to the insect steroid hormone, 20-hydroxy-ecdysone. One of these clones, designated GV1, was studied with respect to cytoskeletal elements. Electron microscopic examination revealed that the microtubule networks became more extensive and organized increasingly parallel following exposure to the insect hormone 20-hydroxy-ecdysone, while inhibitors of microtubules were found to reverse the elongation response in the cells within 2.5 h. Agents affecting microfilaments (cytochalasins B and D) did not specifically alter the elongation response, but treatment with these agents did reveal increased cellular junctions as shown by increased cell aggregate size in hormone-treated cultures exposed to the cytochalasins.

Keywords: Clones; Cytoskeleton; Insect cell cultures; Manduca sexta

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