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Wilehm Roux Arch Dev Biol. 1981 May;190(3):161-167. doi: 10.1007/BF00867803.

Low variability of the protein species synthesized byDrosophila melanogaster embryos.

Wilhelm Roux's archives of developmental biology

Adriano Savoini, Fulvio Micali, Roberto Marzari, Franco de Cristini, Giorgio Graziosi

Affiliations

  1. Istituto di Zoologia ed Anatomia Comparata, Università di Trieste, Trieste, Italy.

PMID: 28305167 DOI: 10.1007/BF00867803

Abstract

Stainable proteins as well as newly synthesized polypeptide chains of proteins extracted fromDrosophila melanogaster embryos were analyzed by two-dimensional gel electrophoresis. The following developmental stages were studied: unfertilized eggs, early nuclear multiplication (25 min average age), late nuclear multiplication (105 min), cellular blastoderm (165 min), gastrula (4 h), mesodermal segmentation (6 h) and muscleattachment (8 h). One hundred and fifty stainable spots were present at all developmental stages and were all also synthesized during development, with the exception of 5 unkown proteins and the three yolk proteins. Out of 400 proteins which were labelled by

Keywords: Actins; Drosophila embryo; Heat-shock proteins; Protein synthesis; Tubulins

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